A-Galactosidases

Defatted soy flour was suspended in water to 8% (v/w) protein, pH was adjusted to 6 by HCl before 640 mg/L Novozyme 415 (a-galactosidase), 10 mg/L Phytase L (Novo Nordisk) and 53 mg enzyme protein/L rhamnogalacturonase B were added. After 4 hours at 50 C the pH was adjusted to 8 by NaOH, and the slurry was centrifuged. The supernatant was pasteurized (85°C, 5 minutes) and freezedried. Protein was measured as 6.25 x Kjeldahl N. Phytate was measured as described in [16], and dietary fibres were analysed as described in [17]. 

The ability of RGase B to solubilise soy fibres is useful for the production of a fibre enriched dietetic soy product. Soy milk has the disadvantages that it has a low fibre content and a high content of phytate and of stachyose and raffinose. To overcome these disadvantages a-galactosidase was used to reduce the amount of stachyose and raffinose, phytase to degrade the phytate, and RGase B to release fibre material. 

Tobacco mosaic virus CP replacement, Second subgenomic promoter (sgp), CP fusion, Readthrough fusion with CP, fusion with cleavage site, fusion with CtxB malaria, rabies virus, M HV, FMDV, HCV, BHV-1, Ps. aeruginosa, neuropeptide, TMOF, allergens, MABs, a-trichosanthin, cytokines a-galactosidase-A, scFvs 11, 16,17, 52, 59-61... 

Human a-galactosidase is a 100 kDa homodimeric glycoprotein. Each 398 amino acid monomer displays a molecular mass of 45.3 kDa (excluding the glycocomponent) and is glycosylated at three positions (asparagines 108, 161 and 184). After administration (usually every second week by a 40 min infusion), the enzyme is taken up by various body cell types and directed to the lysosomes. This cellular uptake and delivery process appear to be mediated by mannose-... 

Fabry s disease a-galactosidase (trihexoceramidase) Trihexosylceramide (gal-gal-glc-cer digalactosylceramide)... 

Luonteri, Elina. Fungal a-arabinofuranosidases and a-galactosidases acting on polysaccharides. 1998. 113 p. + app. 59 p. 

Scheme 4 6 -0-Acyl-lactose (acetyl, propionyl, butyryl) derivatives prepared via the selective enzymatic acylation of lactose by the protease subtilisin were used as acceptors for enzymatic transglycosylations catalyzed by a-D-galactosidase from Talaromyces flavus, forming iso-globotriose a-Gal(l -> 3)-p-Gal-(l -> 4).92 (/) Protease N, pyridine, 37 °C (ii) a-galactosidase from Talaromyces flavus.

Since then it has been established that emulsin is a mixture of enzymes comprising in addition to /3-glucosidase28 the enzymes a-galactosidase, a-mannosidase, /3-glucuronidase and /3-(N-acetyl)-glucosaminidase. Concerning the process of purification of the Rohferment and the mode of differentiation of the constituent enzymes of emulsin, the most informa-... 

Thus, to summarize and to show that the two proteins interact, AH109 yeast cells transformed with the appropriate DNA-BD and AD vectors grow in -W, -L SD media and also in -W, -L, -H, -Ade SD media and yield a-galactosidase and p-galactosidase reporter gene activities. 

Fleet GWJ, Nicholas SJ, Smith PW, Evans SV, Fellows LE, Nash RJ. (1985) Potent competitive inhibition of a-galactosidase and a-glucosidase activity by l,4-dideoxy-l,4-iminopentitols Synthesis of l,4-dideoxy-l,4-imino-D-lyxitol and of both enantiomers of l,4-dideoxy-l,4-iminoarabinitol. 

---