Galactosidase, a- and

In maize-root tips, high specific activities of/ -D-galactosidase, a- and jff-D-glucosidase, N-acetyl-/ -D-glucosaminidase, acid phosphatase, and phosphodiesterase (EC 3.1.4.1) are found in the cell-wall fraction.246... 

Peluso, R., Godwin, S., Carter, B. J., Gregory, R. J., Wadsworth, S. C. and Cheng, S. H. (2004). AAV2 vector harboring a liver-restricted promoter facilitates sustained expression of therapeutic levels of alpha-galactosidase A and the induction of immune tolerance in Fabry mice. Mol. Ther. 9, 231-240. 

Another genetic metabolic disorder is Fabry disease, which is related to a deficiency in the enzyme a-galactosidase A. This enzyme is involved in the metabolism of lipids, and its deficiency leads to the accumulation of lipids in the eyes, kidneys, and nervous and cardiovascular systems. In 2001, the biopharmaceutical called Fabrazyme was approved, which consists of recombinant a-galactosidase A and is produced by genetically modified CHO cells. 

Agalsidase is an enzyme replacement therapy that provides an exogenous source of a-galactosidase A and is used in the treatment of Fabry s disease. 

The release of )S-D-galactosidase from the vesicles of human intestinal brush-border membranes by various enzymes, particularly pancreatic proteases, has been studied. a-o-Galactosidases A and B have been extensively purified from human liver by classical procedures. In contrast to previous reports, it was shown that the A form is not converted into the B form on treatment with neuraminidase, although an apparent transformation of the A form into the B form was revealed by Cellogel electrophoresis. 

Rossi, F., Charlton, C. A., and Blau, H. M. (1997). Monitoring protein-protein interactions in intact eukaryotic cells by O-galactosidase complementation. Proc. Natl. Acad. Sci. USA 94, 8405-8410. 

Kato, K., Hamaguchi, Y., Fukui, H., and Ishikawa, E. (1975b) Enzyme-linked immunoassay. II. A simple method for synthesis of the rabbit antibody-b-D-galactosidase complex and its general applicability. J. Biochem. (Tokyo) 78, 423. 

Since then it has been established that emulsin is a mixture of enzymes comprising in addition to /3-glucosidase28 the enzymes a-galactosidase, a-mannosidase, /3-glucuronidase and /3-(N-acetyl)-glucosaminidase. Concerning the process of purification of the Rohferment and the mode of differentiation of the constituent enzymes of emulsin, the most informa-... 

Exoglycosidases that are useful in carbohydrate degradations include neuraminidases, fucosidases, galactosidases, mannosidases, and aminohexosidases.76,77 An example of the use of degradation by a gly-cosidase in conjunction with methylation analysis is a study on the oc-... 

There are other reporter gene systems, such as j8-galactosidase (a bacterial enzyme), chloramphenicol acetyltransferase (a bacterial enzyme), and aequorin (a jellyfish protein). 

Colorimetric assays for a- and (3-galactosidase reportei gene activities... 

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