A. aerogenes

Glueuronidase E. coli, A. aerogenes Cleavage of P-glucuronidases of alcohols and phenols... 

A specific protein inhibitor for 5 -nucleotidase has been purified from E. coli cell cytoplasm (10, 16). It prevents the action of the enzyme on 5 -AMP, ATP, and UDPG. It also inhibits the hydrolysis of 5 -AMP by the 5 -nucleotidases from A. aerogenes, S. sonnei, and S. typhimurium (10). Other Enterobacteriaceae also possess similar intracellular protein inhibitors (18) which inhibit all hydrolytic activities of the 5 -nucleo-tidase of these organisms. The relevance of this inhibitor protein to the action of the enzyme in vivo is not known. 

The kinetic lability of ferric siderophores requires that transport experiments be performed with molecules bearing separate radioactive labels in the metal and ligand moieties. As coordination compounds the siderophores are thermodynamically stable and kinetically labile. The formation constants are typically 1030. In the case of ferrichrome the exchange half time at pH 6.3 and 37° is about 10 min (57). Published work (58, 59) with doubly labeled ferric schizokinen in Bacillus mega-terium and ferric aerobactin in A. aerogenes as well as a study of ferric enterobactin in E. coli (60) in each instance suggests a synchronous uptake mechanism for iron and ligand. 

Pullulanase is an extracellular enzyme of Aerobacter aerogenes that causes essentially quantitative hydrolysis of pullulan to maltotriose. The enzyme is readily prepared in a crude form that is free from other carbohydrases, and is important in structural studies because it debranches amylopectin and glycogen. When the A. aerogenes is grown in continuous culture, the enzyme is bound to the cells, but it can be released by detergents, and purified by ion-exchange chromatography. This purified enzyme has been crystallized. ... 

When one considers the further metabolism of DDT, the picture becomes more confusing. Wedemeyer 16, 26) has proposed the pathway from DDD to DBP shown in Figure 5. This pathway is similar to that proposed originally for rats by Petersen and Robinson (27). It was developed by detecting the succeeding metabolites after incubating the various proposed intermediates with A. aerogenes. 

Some discrepancies appear also in the study of the steps from DDA to DBP (26). When DDA was incubated with A. aerogenes for seven days, the recovery of DDA decreased from 260 to 47 fig. The recovery of DPM rose to 250 fig at four days and decreased to 10 fig at... 

Fig. 17.4. Spectrogram of characteristic spectra of selected bacteria ( 4) A. aero-genes (strain 1B21) (B) A. aerogenes (strain B-7) (C) E. coli (D) B. subtitis (E) B. cereus (f) P. aeruginosa (G) P. ftuorescens. (Reynolds et ai, 1967.)...

That 2-acetylthiamine pyrophosphate is an intermediate in the oxidative decarboxylation of pyruvate may also be. inferred from (a) the demonstration by Goedde et al. (1961) that the hydroxyethyl group of 2-hydroxyethyl-thiamine pyrophosphate can be converted to acetyl CoA in the presence of a pyruvate oxidation system from yeast mitochondria and (b) the demonstration by Krampitz et al. (1961) that the hydroxyethyl group of 2-hydrox-yethylthiamine pyrophosphate is oxidized to acetate in the presence of fcrricyanide and the acetoin-forming system from A. aerogenes. 

An early indication that the enzymes of the tryptophan biosynthetic pathway were subject to repression control by the end product was a report by Monod and Cohen-Bazire [129] that tryptophan synthetase formation in A. aerogenes was inhibited by tryptophan. Yanofsky [7] described much of the early work on repression of the tryptophan biosynthetic enzymes. 

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