15N nuclear magnetic resonance

Inverse-Detected 15N Nuclear Magnetic Resonance Experiments... 

R.L. Lichter 15N Nuclear Magnetic Resonance, in F.C. Nachod and J.J. Zuckennan, (eds.) Determination of Organic Structures by Physical Methods, Vol. 4. Academic Press, New York, London 1971, chapt. 4. 

Raoul S, Berger M, Buchko GW, Joshi PC, Morin B, Weinfeld M, Cadet J (1996)1 FI, 13C, and 15N nuclear magnetic resonance analysis and chemical features of the two main radical oxidation products of 2 -deoxyguanosine oxazolone and imidazolone nucleosides. J Chem Soc Perkin Trans 2 371-381... 

Knicker, H. (2000a). Biogenic nitrogen in soils as revealed by solid-state 13C and 15N nuclear magnetic resonance spectroscopy. J. Environ. Qual. 29,715-723. 

Mathias LJ, Davis RD, Jarrett WL.(1999). Observation of a- and g-crystal forms and amorphous regions of nylon 6-clay nanocomposites using solid-state 15N nuclear magnetic resonance. Macromolecules 32 7958-60. 

Nuclear magnetic resonance spectroscopy for /V-acyloxy-/V-alkoxyamides 13C NMR spectroscopy, 56-58 15N NMR spectroscopy, 58-59 dynamic 1H NMR spectroscopy, 59 Nucleophilic substitution (SN2) reactions, /V-acyloxy-/V-alkoxyamidcs, 70-90 alcoholysis reactions, 89-90 with aromatic amines, HERON reactions, 70-74... 

Probably, one of the most valuable advances in this field has dealt with the first chemoenzymatic synthesis of the stable isotope-enriched heparin from a uniformly double labelled 13C, 15N /V-acetylheparosan from E. coli K5. Heteronuclear, multidimensional nuclear magnetic resonance spectroscopy was employed to analyze the chemical composition and solution conformation of N-acety 1 hcparosan, the precursors, and heparin. Isotopic enrichment was found to provide well-resolved 13C spectra with the high sensitivity required for conformational studies of these biomolecules. Stable isotope-labelled heparin was indistinguishable from heparin derived from animal tissues and might be employed as a novel tool for studying the interaction of heparin with different receptors.30... 

Nuclear magnetic resonance (NMR) is a widely utilized technique, which detects the reorientation of nuclear spins in a magnetic field. It can potentially be used to determine the 3-D structure of the protein itself, as well as supplying information on kinetics and dynamics, ligand binding, determination of pK- values of individual amino acid residues, on electronic structure and magnetic properties, to mention only some of the applications. In addition, it can be selectively applied to specific nuclei—1H, 13C, 15N, 19F (often substituted for H as a... 

For what kinds of substances can we expect nuclear magnetic resonance absorption to occur Magnetic properties always are found with nuclei of odd-numbered masses, H, 13C, 15N, 170, 19F, 31P, and so on, as well as for... 

Nuclear magnetic resonance (NMR) spectroscopy has been used to directly observe varied persistent superelectrophilic species. Although H and 13C NMR have been the most often used techniques, there have also been applications of 15N, 170, and 19F NMR in their structural characterization. Coupled with theoretical computational methods capable of estimating NMR chemical shifts, these studies have been very useful in the study of superelectrophiles. 

DiCosty, R. J., Weliky, D. P, Anderson, S. J., and Paul, E. A. (2003). 15N-CPMAS nuclear magnetic resonance spectroscopy and biological stability of soil organic nitrogen in whole soil and particle-size fraction. Org. Geochem. 34,1635-1650. 

This chapter describes protocols for preparing 15N-labeled proteins (ubiquitin is used as an example) using Escherichia coli cells (with purification) and the wheat germ cell-free system (without purification). A comparison of I I-15N heteronuclear single-quantum coherence (HSQC) spectra of yeast ubiquitin prepared using each method indicates that this wheat germ cell-free system may be used for rapid nuclear magnetic resonance analyses of proteins without purification. 

Duthaler, R.O. and Roberts, J.D., Nitrogen-15 nuclear magnetic resonance spectroscopy solvent effects on the 15N chemical shifts of saturated amines and their hydrochlorides, J. Magn. Reson., 34, 129, 1979. 

Callies, R Altenburger, R., Abarzua, S Mayer, A., Grimme, L.H., Leibfritz, D. (1992). In situ nuclear magnetic resonance of 15N pulse labels monitors different routes for nitrogen assimilation. Plant Physiol. 100, 1584-1586. 

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