Yeast carboxymethylation

Inactivation of alcohol dehydrogenase from yeast with 14C-labeled [3-(3-bromoacetylpyridinio)-propyl]-adenosine pyrophosphate followed by oxidation showed the presence of 1-carboxymethyl histidine66. After inactivation of the enzyme with labeled [3-(4-bromoacetylpyridinio)-propyl]-adenosine pyrophosphate followed by oxidation, S-carboxymethyl cysteine was identified in the protein. In the case of glyceraldehyde-3-phosphate dehydrogenase, treatment with either coenzyme analogue leads to the modification of the cysteine residue. Treatment with [14C]nicotinamide-5-bromo-4-methylimidazole dinucleotide did not reveal any modified amino-acid-residues. The labeled nicotinamide residue split off during the recovery of the inactivated enzyme. Attempts to synthesize an inactivator labeled with a 14C-acetyl residue did not give satisfactory yields. If the enzyme-coenzyme derivative was treated with tritiated sodium boron hydride, tritium could be introduced (Fig. 22). Studies with... 

Carboxymethyl-agarose has been treated with 5-(2-carboxyethyl)-6-azauridine 5 -phosphate using a carbodi-imide mediator, to provide an efficient affinity column for purification of orotidine-5 -phosphate decarboxylase from yeast. ... 

Bakers yeast extract Baker s yeast glycan Calcium acetate Calcium alginate Calcium carboxymethyl cellulose Calcium carrageenan Calcium chloride Calcium furcelleran Calcium gluconate Calcium glycerophosphate Calcium lactate Calcium sulfate Calcium sulfate dihydrate Carboxymethylcellulose sodium Carrageenan (Chondrus crispus) Carrageenan (Chondrus crispus) extract Cassia gum Cellulose Chitin Chitosan Corn (Zea mays) starch Corn starch, waxy Corn syrup Dextrin Distarch phosphate Ethyl hydroxyethyl cellulose Ferrous sulfate anhydrous Food starch, modified ... 

ZUlli F., Suter F., Biltz H., Nissen H.P., Birman M., Carboxymethylated P(l,3)-glucan, a beta glucan from baker s yeast helps protect skin, Cosmet. Toiletries, 111(12), 1996, 91-98. 

Ribonuclease B, globin, R17-bacteriophage coat protein, carboxymethylated protein, subunits from BI component of TYMV tryptic digest of glyceraldehyde 3-phosphaie dehydrogenase of yeast Dipeptides with Leu/Ile as N-terminal residues Several di- and tri-peptides (see Table 2)... 

The first paper in a new journal devoted to carbohydrates, nucleosides, and nucleotides has reviewed the mechanisms of nucleoside synthesis by condensation reactions. 5-Carboxymethyl-2 -0-methyluridine has been found as a component of yeast tRNA, and the unknown nucleoside Q present in some tRNAs has been identified as 7-(4,5-m-dihydroxy-l-cyclopenten-3-ylaminomethyl)-7-deaza-guanosine (350). Syntheses of 9-j8-D-glucopyranosyl and 7- and 9-/5-D-gluco-furanosyl derivatives of zeatin and 6-benzylaminopurine have been reported ... 

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