Whole-cell biocatalysts mutants

The invention of new methods of protein engineering in the past decade has led to the construction of an abundance of P450 mutants with new tailored properties. Recent major achievements include significant increases in productivities, yields, and rates of catalytic turnover as well as modification of substrate specificity and efficient multistep reactions in whole-cell biocatalysts, coming one step closer to the technical application of cytochrome P450 monooxygenases. 

Application cf Whole-Cell Biocatalysts Possessing Mutant PARs and LSADH 1165... 

When immobilized whole cells were first introduced many researchers hesitated to use them because of their non-specificity and the expected by-product formation. Efforts have been made to utilize as many as possible of the potential advantages offered by immobilized whole cells, and at the same time to make the biocatalyst specific. In such endeavours selective enzyme denaturation, cell membrane modification (9,32,33) and specific mutants for example, have been used. 

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