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Weak anion exchange HPLC

Fig. 3,2. Separation of four arsenic species by weak anion-exchange HPLC with ICP-MS detection. Each peak corresponds to 2 ng of As. Sample size, 200 /il. Mobile phase, 30% methanol-15 mM NH4H2PO4-2 mM CH3COONH4. Flow-rate, 1 ml min for 6 min followed by 2 ml min . Monitoring, miz = 75. Reproduced from ref (73), J. Anal. At. Spectrom., 4 (1989) 279, by permission of The Royal Society of Chemistiy. Fig. 3,2. Separation of four arsenic species by weak anion-exchange HPLC with ICP-MS detection. Each peak corresponds to 2 ng of As. Sample size, 200 /il. Mobile phase, 30% methanol-15 mM NH4H2PO4-2 mM CH3COONH4. Flow-rate, 1 ml min for 6 min followed by 2 ml min . Monitoring, miz = 75. Reproduced from ref (73), J. Anal. At. Spectrom., 4 (1989) 279, by permission of The Royal Society of Chemistiy.
Figure 7.27. HPLC analysis of a protein mixture using weak anion-exchange chromatography using a polymer-based column and UV detection at 230nm. Chromatogram courtesy of PerkinElmer, Inc. Figure 7.27. HPLC analysis of a protein mixture using weak anion-exchange chromatography using a polymer-based column and UV detection at 230nm. Chromatogram courtesy of PerkinElmer, Inc.
Figure 7.33. HPLC analysis of nuclei acids (pBR322-DNA HAE-III digest) using gradient weak-anion exchange chromatography on a column packed with 2.5-pm nonporous polymer support (weak anion exchange) with UV detection at 260nm. Reprinted with permission from reference 40. Figure 7.33. HPLC analysis of nuclei acids (pBR322-DNA HAE-III digest) using gradient weak-anion exchange chromatography on a column packed with 2.5-pm nonporous polymer support (weak anion exchange) with UV detection at 260nm. Reprinted with permission from reference 40.
Cellulolytic enzymes (Trichoderma viride--reesei) HPLC weakly anion exchange groups bound to silica gel Montenecourt et al. [349]... [Pg.252]

Nucleic acid constituents HPLC silica based weak anion exchange column Edelson et al. [390]... [Pg.255]

Several HPLC stationary phases are used for the separation of glycans, including amide-bonded hydrophilic interaction (HILIC), reversed phase (RP), weak anion exchange (WAX), and porous graphitized carbon (PGC). Due to the heterogeneous nature and complexity of many glycan populations, analysis may require the use of several orthogonal methods [7],... [Pg.189]

Dabre R, Azad N, Schwaemmle A, Laemmerhofer M, Lindner W. Simultaneous separation and analysis of water- and fat-soluble vitamins on multi-modal reversed-phase weak anion exchange material by HPLC-UV. J. Sep. Sci. 2011 34 761-772. [Pg.1624]

The hydrophilic silica-based diol packings have been modified by derivation through some of the diol groups with carboxymethyl and diethy-laminoethyl functions to make weak anionic and cationic protein size-separation columns. These provide the HPLC equivalent of the CM- and DEAE-cellulose columns used in protein purification on open columns and are used with the same type of buffers to provide ion exchange purifications of proteins. [Pg.101]

HPLC weakly basic anion exchange column... [Pg.254]

HPLC weakly basic pellicular anion exchanger... [Pg.254]


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