Water spinning

B. Apparent Paradox of Hydrophobic Proteins and Water Spinning ... 21... 

Fig. 3. Schematic representation of the topological space of hydration water in silica fine-particle cluster (45). The processes responsible for the water spin-lattice relaxation behavior are restricted rotational diffusion about an axis normal to the local surface (y process), reorientations mediated by translational displacements on the length scale of a monomer (P process), reorientations mediated by translational displacements in the length scale of the clusters (a process), and exchange with free water as a cutoff limit.

Fig. 10. The water spin-lattice relaxation rates as a function of magnetic field strength represented as the Larmor frequency for packed samples of calibrated porous glass beads with pore diameter of 75 A at several temperatures. The solid lines are best fits to the theory (67).

Fig. 14. Measured water spin-lattice relaxation rates of a hydrated mortar at w/c = 0.38 as a function of the proton Larmor frequency, for different duration of hydration Oh 34 min ( ), 7h 27 min (O), 8h 45 min ( ), and 9h 40 min ( x ), upwards. The insert represents the data obtained after a hydration time of lOh 32 (+), the labels for the two axis are equivalent to those of the main figure. The continuous lines correspond to the best fits to the theory.

The importance of the magnetic coupling is easily seen in Fig. 17 which shows two water proton MRD profiles for serum albumin solutions at the same composition (89). The approximately Lorentzian dispersion is obtained for the solution, and reports the effective rotational correlation time for the protein. The magnetic coupling between the protein and the water protons carries the information on the slow reorientation of the protein to the water spins by chemical exchange of the water molecules and protons between the protein and the bulk solution. When the protein is cross-linked with itself at the same total concentration of protein, the rotational motion of the protein... 

Extract the chlorophyll from the chloroplasts by mixing, in a conical centrifuge tube, 0.05 mL of well-mixed chloroplast suspension with 9.9 mL of 80% acetone in water. Spin in a tabletop centrifuge for 10 minutes. Transfer the supernatant to a glass cuvette and read the absorbance at 652 nm using 80% acetone in water as reference. Calculate the concentration of chlorophyll in the chloroplast suspension using Equation E9.3. 

Reaction (76) has been believed to ruled out in a kinetic analysis of the Fenton reaction by EPR spin trapping (Mizuta et al. 1997) [a caveat is the observation that in water spin traps may be oxidized to the OH-adduct via the spin trap radical cation by strong oxidants (Eberson and Persson 1997 von Sonntag et al. 2004)], but reaction (77), already suggested earlier (Rush and Koppenol 1987), was required to account for their data. 

Picullel L, Halle B (1986) Water spin relaxation in colloidal systems. Part 2., 70 and 2H relaxation in protein solutions. J Chem Soc Faraday Trans I 82 401-414... 

The DNA can he seen directly in the CsCl gradient by reversibly staining with ethidium bromide (Firtel and Bonner 1971). Solid CsCl is added to the DNA solution to make the density up to about 1.55 g ml (0.97 g CsCl to each 1 ml original DNA solution) followed hy 1/20 volume of ethidium bromide solution (10 mg/ml in water). Spinning time is 48 hr at 40,000 rev/min. RNA and... 

StiU, Tikhonov and Volkov repeatedly observed the above shift in the ratio of water spin isomers after contacting water vapor with the polymeric adsorbent both under dynamic (Fig. 10.19) and static conditions [30]. Activated carbons, zeolites, and sifica gels generated similar effects. The adsorbed water was strongly para-enriched. Interestingly, water samples enriched in the ortho- or para-isomers were found to be stable in the form of ice, but equilibrated to the ratio of 3 1 in the hquid state at room temperature within 20—30 min. As the separation takes place at the... 

FIGURE 12.26 Transmission electron micrograph of a cross section of acrylic fiber removed from a 55°C dimethylacetamide water spin bath and freeze-dried. Magnification, 20,000 x. 

Pour off the supernatant and resuspend the cell pellet in 5 mL of sterile water. Spin at 1500g for 5 mm to harvest the cells. 

Fig. 3.3a,b. GRE in phase and out of phase, a GRE in phase (TE 4.4 ms) no focal lesions are visible, b GRE out of phase (TE 2.2 ms) some focal fat infiltrations are visible as hypointense lesions, because fat and water spins are in opposed phase, then their signal cannot be summed up... 

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