Viral culture

The gold standard for diagnosis of influenza is viral culture. 

Transmission between Influenza-like symptoms Viral culture Oseltamivir and... 

Confirmatory laboratory testing has been limited to viral cultures with subsequent immunoassay testing, which has limited its use, making reliance on history and clinical signs for diagnosis. A new in-office test for the adenovirus has been marketed. The RPS Adeno Detector is a 10-minute, in-office, lateral flow immunoassay that detects the presence of adenovirus in suspected... 

Although not typically used, laboratory tests are available to help diagnose HSK in equivocal cases. One of the most reliable and festest tests is the Herpchek , which is an enzyme immimoassay test that yields results in 1 day. Additional laboratory tests include viral culture microbio-logic studies, enzyme-linked virus inducible system, and polymerase chain reaction detection. 

A 19-month-old girl was immunized against Varicella at 15 months of age and later developed zoster infection (18). Viral cultures from various lesions isolated Varicella zoster virus. The Oka vaccine strain was revealed by polymerase chain reaction. 

Chonmaitree T, Menegus MA, Powell KR. The clinical relevance of CSF viral culture. A two-year experience with aseptic meningitis in Rochester, NY. JAMA 1982 247 1843-7. 

Persons DL, Moore JA, Eishback JL. Comparison of polymerase chain reaction, DNA hybridization, and histology with viral culture to detect cytomegalovirus in immunosuppressed patients. Mod Pathol. 1991 4 149-152. 

Cazacu, A. C., Demmler, G. J., Neuman, M. A., Forbes, B. A., Chung, S. Y., Greer, J., Alvarez, A. E., Williams, R., and Bartholoma, N. Y. (2004) Comparison of a new lateral-flow chromatographic membrane immunoassay to viral culture for rapid detection and differentiation of influenza A and B viruses in respiratory specimens. J Clin Microbiol. 42, 3661-3664... 

An alternative approach involves the use of viral vectors, in which plant viruses are geneticalfy modified to encode the foreign genes. Plants are subsequently infected with the modified virus and thus act as viral culture vessels . By this means, very large quantities of recombinant proteins can be generated... 

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