Vesicle proximity effects

The aforementioned results refer to unilamellar membrane models but essentially similar results are obtained in multilamellar vesicles, though the kinetics are more complex in such systems. The numerical values observed in these model membranes simply show that one or more of the aforementioned factors arise however, in the in vivo situation, the preeminent effect is unknown but may well be the proximity of the hydroxyl group to the water interface. 

The effects of CPH-treatment of rats (1200 mg/ kg/d for 3d) on the polypeptide composition of renal brush border from the proximal tubule cells enzymatic activities and transport systems of the brush border membrane vesicles (BBMV) were investigated [77]. The results of these studies showed that CPH-treatment induces a 20-30% decrease in the specific activities of renal brush border enzymes leucine aminopeptidase and D-glutamyltransferase. SDS-gel electrophoresis showed that CPH-treatment induced a decrease of the intensity of 3 brush border polypeptides of molecular weights of 72,000, 58,000 and 39,000 [77]. 

As long as the radial extension of 1st is mudi smaller than Oa bz [0, is the std.dev. of (a)] the Lorentz factor l/b will be a reasonable proximation for (1.12), but in the general case it is invalid and we have to know the exact three-dimensional form of 1st ih reciprocal space in order to evaluate lexp. Before we evaluate the general three-dimensional expression of via Qgx, we will demonstrate an additional effect of membrane undulations on the integral (1.12). For the sake of simplicity we assume stacks consisting of only one vesicle with a deltalike distribution of inter-membrane distances. In Chaps. 2 and 3 Igx was restricted to a delta-cylinder around the hz-axis and as a consequence, all our knowledge of membrane structure was based on the projection of the vesicle-layer density onto the z-axis ... 

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