Membrane vesicle brush border

In eukaryotes there is also evidence that Met(O) is actively transported. It has been reported that Met(O) is transported into purified rabbit intestinal and renal brush border membrane vesicles by a Met-dependent mechanism and accumulates inside the vesicles against a concentration gradient102. In both types of vesicles the rate of transport is increased with increasing concentrations of Na+ in the incubation medium. The effect of the Na+ is to increase the affinity of Met(O) for the carrier. Similar to that found in the bacterial system, the presence of Met and other amino acids in the incubation medium decreased the transport of Met(O). These results suggest that Met(O) is not transported by a unique carrier. 

Biochemical studies of plasma membrane Na /H exchangers have been directed at two major goals (1) identification of amino acids that are involved in the transport mechanism and (2) identification and characterization of the transport pro-tein(s). To date, most studies have been performed on the amiloride-resistant form of Na /H" exchanger that is present in apical or brush border membrane vesicles from mammalian kidney, probably because of the relative abundance of transport activity in this starting material. However, some studies have also been performed on the amiloride-sensitive isoform present in non-epithelial cells. 

Subsequently, proteolytic fragments of the rabbit renal 25-kDa amiloride-binding protein were micro-sequenced and found to have high sequence homology with rat and human NAD(P)H quinone oxidoreductase. Indeed, enzymatic assays revealed that renal brush border membrane vesicles contain significant NADPH quinone oxidoreductase activity. Presumably NAD(P)H quinone oxidoreductase coincidentally binds amiloride analogs with the same rank order as the Na /H exchanger [39]. 

Temperature 20°C, equilibrium dialysis, small unilamellar vesicles (DOPC), 0.1 M KC1 [382]. Centrifugation method (15 min, 150,000 g), brush-border membrane vesicles [433]. 

Alcorn, C. J. Simpson, R. J. Leahy, D. E. Peters, T. J., Partition and distribution coefficients of solutes and drugs in Brush Border membrane vesicles, Biochem. Pharmacol. 45, 1775-1782 (1993). 

Mucosal brush border membrane vesicles and basolateral membrane vesicles can be isolated to study solute uptake across specific enterocyte boundaries. These more isolated vesicle systems allow for investigation of solute transport across a particular membrane barrier and permit separation of membrane trans-... 

H Yuasa, D Fleisher, GL Amidon. Noncompetitive inhibition of cephradine uptake by enalapril in rabbit intestinal brush-border membrane vesicles An enalapril specific inhibitory binding site on the peptide carrier. J Pharmacol Exp Ther 269 1107-1111, 1994. 

N Piyapolrungroj, C Li, RL Pisoni, D Fleisher. Cimetidine transport in brush-border membrane vesicles from rat small intestine. J Pharmacol Exp Ther 289 346-353, 1999. 

V Ganapathy, FH Leibach. (1990). Peptide transport in intestinal and renal brush border membrane vesicles. Life Sci 30 2137-2146. 

Ganapathy, V. and F. H. Leibach. Role of pH gradient and membrane potential in dipeptide transport in intestinal and renal brush-border membrane vesicles from the rabbit. Studies with L-camosine and glycyl-L-proline. J. Biol. Chem. 1983, 258, 14189-14192. 

Poschet, J. F., S. M. Hammond, and P. D. Fairclough. Characterisation of penicillin-G uptake in rabbit small-intestinal brush-border membrane vesicles. Biochim. Biophys. Acta 1996, 1278, 233-240. 

Kitagawa, S., J. Takeda, and S. Sato. pH-dependent inhibitory effects of angiotensin-converting enzyme inhibitors on cefroxadine uptake by rabbit small intestinal brush-border membrane vesicles and their relationship with hydrophobicity and the ratio of zwitterionic species. Biol. Pharm. Bull. 1999, 22, 721-724. 

Hashimoto, N., et al. Renin inhibitor transport mechanism in rat small intestinal brush-border membrane vesicles. Pharm. Res. 1994, 11, 1448— 1451. 

Takano, M., et al. Bestatin transport in rabbit intestinal brush-border membrane vesicles. Biochem. Pharmacol. 1994, 47, 1089-1090. 

Ishizawa, T., et al. Mechanisms of intestinal absorption of the antibiotic, fosfomydn, in brush-border membrane vesicles in rabbits and humans./. Pharmacobiodyn. 1992, 25, 481-489. 

F. Ushigome, N. Koyabu, S. Satoh, K. Tsukimori, H. Nakano, T. Nakamura, T. Uchiumi, M. Kuwano, H. Ohtani, and Y. Sawada. Kinetic analysis of P-glycoprotein-mediated transport by using normal human placental brush-border membrane vesicles. Pharm Res. 20 38-44 (2003). 

Said, H.M., Redha, R., and Nylander, W., A carrier-mediated Na+ gradient-dependent transport for biotin in brush-border membrane vesicles, Am. ]. Physiol., 253, G631, 1987. 

Kobayashi, M., Suruga, S., Takeuchi, H., Sugawara, M., Iseki, K., and Miyazaki, K. A structure-relationship study of the uptake of aliphatic polyamine compounds by rat intestinal brush-border membrane vesicles, / Pharm. Pharmacol., 49(5) 511-515, 1997. 

Bertolo, R. F. P., Bettger, W. J., and Atkinson, S. A. (2001). Divalent metals inhibit and lactose stimulates zinc transport across brush border membrane vesicles from piglets.. Nutr. 

Gunshin, H., Noguchi, T., and Naito, H. (1991). Effect of calcium on the zinc uptake by brush border membrane vesicles isolated from the rat small intestine. Agric. Biol. Client. 55, 2813-2816. 

Roth-Bassell, FI. A., and Clydesdale, F. M. (1991). The influence of zinc, magnesium, and iron on calcium uptake in brush border membrane vesicles. /. Am. Coll. Nutr. 10, 44- 9. 

Ileal oleic acid uptake. Hydrogenated oil, administered to rats at a dose of 5 g/100 g of diet, produced saturable kinetics in ileal brush border membrane vesicles = 0.23... 

Jejunal oleic acid uptake. Hydrogenated oil, administered to rats at a dose of 5 g/100 g of diet, produced saturable kinetics in jejunal brush border membrane vesicles = 0.15 + 01 pmol/mgprotein/5 minutes, and K = 136 + 29.1 nmol for controls, and Vmax =03 + 01 pmol/mg protein/5 minutes and K = 124.5 + 72.6 nmol for the coconut oil-fed group . 

CN198 Prieto, R. M., W. Stremmel, C. Sales, and J. A. Tur. Effect of dietary fatty acids on jejunal and ileal oleic acid uptake by rat brush border membrane vesicles. Eur J Med Res 1996 1(7) 355-360. 

Cermak, R., Landgraf, S., and Wolffram, S., Quercetin glucosides inhibit glucose uptake into brush-border-membrane vesicles of porcine jejunum, Br. J. Nutr., 91, 849, 2004. 

Welsch, C.A., Lachance, P.A., and Wasserman, B.P., Dietary phenolic compounds inhibition of Na -dependent o-glucose uptake in rat intestinal brush border membrane vesicles, J. Nutr., 119, 1698, 1989. 

Hashimoto, T., et al. Improvement of intestinal absorption of peptides Adsorption of Bl-Phe monoglucosylated insulin to rat intestinal brush-border membrane vesicles. Eur J Pharm Biopharm 50 197. 

Langguth, P., et al. 1994. Metabolism and transport of the pentapeptide metkephamid by brush-border membrane vesicles of rat intestine. J Pharm Pharmacol 46 34. 

Luessen, H.L., et al. 1996. Mucoadhesive polymers in peroral peptide drug delivery. V. Effect of poly(acrylates) on the enzymatic degradation of peptide drugs by intestinal brush border membrane vesicles. Int J Pharm 141 39. 

Excretion Epithelial cells Urine Brush border membrane vesicles, transporter expression system... 

Small intestine Uptake Digestive tract Epithelial cells Everted sac, Ussing-chamber experiments using intestinal epithelium, brush border membrane vesicles, Caco-2 cells monolayer, transporter expression system... 

Brush border membrane vesicles (BBMV), prepared from rat intestine, were used to elucidate the function of P-gp in this organ and to show that the subcellular distribution of P-gp is localized to the AP membrane (411). The differences in P-gp-mediated efflux seen in the ileum, jejunum, and duodenum of... 

---