Vasodilator-stimulated phosphoproteins

VASP, vasodilator stimulated phosphoprotein hsp27, heat shock protein hsp27 LASP, LIM and SH3 domain containing protein Tx, thromboxane IP3, inositol triphosphate ADP, adenosine 5 -diphosphate. Adapted from Refs. [44] and [116]. 

Consensus docking sites for actin-based motihty, defined by the oligoproline modules in Listeria monocytogenes ActA surface protein and human platelet vasodilator-stimulated phosphoprotein (VASP). Analysis of... 

Chakrabarty et al. discovered that the host cell protein vasodilator-stimulated phosphoprotein (VASP) concentrates at the back of motile Listeria at the bacteria-tail junction. VASP docks onto the surface of Listeria by binding to the oligoproline sequences of ActA concentrated on the rearward pole of moving bacteria. In uninfected host cells, VASP is normally found in focal con-... 

Fig. 4. The structure of human vasodilator-stimulated phosphoprotein (VASP) is that of a parallel chain, four-stranded coiled-coil (Kuhnel et al., 2004). The sequence contains a pair of 15-residue repeats, resulting in the formation of a right-handed coiled coil with a pitch length of 18.5 nm, a value directly comparable to that seen in left-handed two-stranded coiled coils. Figure courtesy of Sergei Strelkov.

Zhuang S, Nguyen GT, Chen Y, Gudi T, Eigenthaler M, Jarchau T, Walter U, et al. (2004) Vasodilator-stimulated phosphoprotein activation of serum-response element-dependent transcription occurs downstream of RhoA and is inhibited by cGMP-dependent protein kinase phosphorylation. J Biol Chem 279 10397 407... 

PGI2 exerts its inhibitory activity by increasing cyclic adenosine monophosphate (cAMP) formation via adenylate cyclase, which stimulates phosphorylation of a cytoskeleton integrin protein-associated vasodilator-stimulated phosphoprotein (VASP), which prevents GPIIb Ilia activation (16). 

There are three important ADP receptors on the platelet surface. The P2X, is related with the rapid influx of calcium into the cytosol the P2Y1 mediates mobilization of calcium through activation of phospholipase C and shape change, and the P2Y12 receptor is coupled to adenyl cyclase inhibition mediated by a G-protein with subsequent decrease of the cyclic adenosine monophosphate (cAMP). The decrease of cAMP stimulates dephosphorylation of vasodilator-stimulated phosphoprotein that is closely correlated with the GPIIb/llla activation. Thienopyridines compounds promote platelet inhibition mainly by blocking the P2Y12 receptor. 

Barragan R Bouvier JL, Roquebert PO, et al, Resistance to thienopyridines clinical detection of coronary stent thrombosis by monitoring of vasodilator-stimulated phosphoprotein phosphorylation. Catheter Cardiovasc Interv 2003 59 295-302. 

Aszodi A, Pfeifer A, Ahmad M et al. (1999) The vasodilator-stimulated phosphoprotein (VASP) is involved in cGMP-and cAMP-mediated inhibition of agonist-induced platelet aggregation, but is dispensable for smooth muscle function. EMVBO J 18 37—48... 

Hauser W, Knobeloch KP, Eigenthaler M et al. (1999) Megakaryocyte hyperplasia and enhanced agonist-induced platelet activation in vasodilator-stimulated phosphoprotein knockout mice. Proc Natl Acad Sci USA 96 8120-8125... 

Butt, E., Abel, K., Krieger, M., Palm, D., Hoppe, V., Hoppe, J., Walter, U. (1994). cAMP- and cGMP-dependent protein kinase phosphorylation sites of the focal adhesion vasodilator-stimulated phosphoprotein (VASP) in vitro and in intact human platelets. J. Biol. Chem. 

Phosphorylation of the vasodilator-stimulated phosphoprotein regulates its interaction with actin. 

Gurbel PA, Bliden KP, Etherington A, Tantry US. Assessment of clopidogrel responsiveness measurements of maximum platelet aggregation, final platelet aggregation and their correlation with vasodilator-stimulated phosphoprotein in resistant patients. Thromb Res 2007 121 107—15. 

J. (2006) The bundling activity of vasodilator-stimulated phosphoprotein is required for filopodium formation. Proc. Natl. Acad. Sci. 

Enabled/vasodilator-stimulated phosphoproteins (Ena/VASP) are a structurally conserved family found in vertebrates, invertebrates, and Dictyostdium discoidmm cells. Ena/VASP proteins were shown... 

Hiittelmaier S, Harbeck B, Steffens O, Messerschmidt T, Illenberger S, Jockusch BM (1999) Characterization of the actin binding properties of the vasodilator-stimulated phosphoprotein VASP. FEBS Lett 451 68-74 Hussey PJ, Ketelaar T, Decks MJ (2006) Control of the actin cytoskeleton in plant cell growth. Annu Rev Plant Biol 57 109-125... 

Major, T.C., Handa, H., Brisbois, E.J., Reynolds, M.M., Annich, G.M., Meyerhoff, M.E., Bartlett, R.H., 2013. The mediation of platelet quiescence by NO-releasing polymers via cGMP-induced serine 239 phosphorylation of vasodilator-stimulated phosphoprotein. 

---