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Variable region gene libraries

The isolation of an antibody is an extremely useful first step toward understanding the function of the protein to which it binds. scFvs derived from phage antibody libraries have been used in immunofluorescence, immunoprecipitation, fluorescence-activated cell sorting, Western blotting, and inhibition of function studies, both in vivo, in tissue culture cells, and in vitro. In this sense, they can essentially be used in the same way as conventional hybridoma-derived antibodies. They have the advantage, however, that the genes for the variable regions are cloned simultaneously with selection. This allows the fusion of functional elements, such as dimerization domains, effector or detector functions to selected scFvs [38], the re-creation of complete... [Pg.463]

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See also in sourсe #XX -- [ Pg.25 ]

See also in sourсe #XX -- [ Pg.25 ]



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Gene variability

Region Variability

Variable genes

Variable region gene

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