Vanillin Pseudomonas putida

A schematic flowsheet of the fermentation unit with integrated MBSE and MBSS circuit for recovery of acid(s) product from the fermentation broth is presented in Figure 23.5. Martak et al. [73] ran a semicontinuous fermentation of lactic acid with Rhizopus arrhizus with a periodical bleed and feed operation without a decrease in LA productivity for 152 h. Such a process could be integrated with separation of lactic acid, for example, by MBSE studied in ref. [74] or by pertraction [44,45], Recovery of vanilline from a fermentation broth is presented in ref. [75] aiming at formation of an integrated system. A combination of MBSE of phenol from saline solution in HF contactor with bioreactor with Pseudomonas putida to remove phenol is studied in ref. [76],... 

Graf, N. and Altenbuchner, J. (2014) Genetic engineering of Pseudomonas putida KT2440 for rapid and high-yield production of vanillin from ferulic acid. Appl. Microbiol Biotechnol, 98 (1), 137-149. 

Yamada M, Okada Y, Yoshida T, Nagasawa T (2008) Vanillin production using Escherichia coli cells over-expressing isoeugenol monooxygenase of Pseudomonas putida. Biotechnol Lett 30 665-670... 

A comprehensive screening for vanillin and/or vanillic acid production activities in various bacterial strains allowed the identification of many promising candidates from different species, the best of which Pseudomonas putida IE27) afforded a productivity of 16.1 g 1 under optimized conditions [41]. 

Yamada, M., Okada, Y., Yoshida, T., and Nagasawa, T. (2007) Biotransformation of isoeugenol to vanillin by Pseudomonas putida IE27 cells. Appl. Microbiol. Biotechnol, 73, 1025-1030. 

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