UV-shadowing

Use a UV lamp and a silica plate as a screen to detect the RNA bands by UV shadowing. 

Full-length RNA was detected by UV-shadowing, eluted from the gel by the crush and soak method [14], and ethanol-precipitated. 

Nucleic acids can be visualized by ethidium bromide staining, UV shadowing, or phosphorimaging of radioactive samples. A sterile scalpel should be used to excise the separated product which can then be eluted by electrophoresis (1 x Tris-borate, pH 8.3) into a 30kDa molecular weight cutoff (MWCO) filter (Millipore). The product is concentrated by centrifugation, dialyzed, and resuspended in the buffer of choice. The yield of nucleic acid is typically 75 %, and ethanol precipitation is not needed. 

Analyze the yield by loading 2 pi of each reaction on a denaturing PAGE (8% acrylamide, 8 M urea) and visualizing by UV shadowing or staining with 0.1% toluidine blue solution. 

Hand-held UV shadowing lamp (VWR Scientific), razor blades, ding-film, kimwipes, ethanol and MilliQ grade distilled water. 

Wrap the gel using plastic wrap, as described previously, and detect the DNA by UV shadowing. The top band, which corresponds to the aptamer strand, is then excised with a sterile razor blade and placed into a 1.5 mL centrifuge tube. 

Figure 6 A small G-quadruplex ligand accelerates quadruplex formation, (a) Gel electrophoresis experiment. 100 [iM of TG3T was incubated at 4°C for 4.5 h in a pH 7.2 10 mM lithium cacodylate buffer supplemented with 110 mM KCl, in the presence of various concentrations (0-100 tM) of a specific quadruplex ligand, 360A. Samples were then loaded on a 20% acrylamide TBE IX gel supplemented with 20 mM KCl. Nucleic acids were revealed by UV-shadow using a 254 nm UV lamp. A retarded band reveals the formation of a quadruplex the presence of the quadruplex ligand induces a further retardation. (b) Isothermal (4°C) absorbance measurements at 295 nm of 31 vM TG4T in the absence (circle, left Y-scale) or presence (squares, right Y-square) of 10 iM 360A in a pH 7.2 10 mM lithium cacodylate buffer supplemented with 110 mM NaCl...

In order to reduce the chances of disease in the hatchery, disinfection of the inlet water is crucial. Disinfection can reduce the amount of fish pathogens typically as much as 99.9 % (log 3), although complete sterilization is practically impossible. The most common system is ultraviolet (UV), but it is also possible to use ozone (Summerfelt, 2003 Masters et a/., 2008). Disinfection should be carried out after particle removal in order to avoid any negative influence from the particles during disinfection, UV shadowing, or unnecessary reduction of the ozone concentration. 

Standard UV equipment and clear coats cannot ensure curing of coating in the shadowed areas. 

Figure 5.9. Functionalization of GaN using the UV-photoinduced reaction with alkenes. Using a shadow mask for the irradiation allows for patterned functionalization. In this study, the alkyl terminated layer was later functionalized with DNA. Figure reproduced with permission from Ref. [152]. Copyright...

Figure 9 shows the spectra obtained from the application of the action spectrum for the global, diffuse and direct irradiances for the same case. It can be seen the change in the spectra and the increase of the importance of diffuse irradiance relative to global. In this context, it can be expected that UV diffuse radiation have an important role in the erithema formation. This result supports the idea that shadow is not enough to avoid a sunburn and that additional protection measures must be taken in order to avoid also the exposition to diffuse radiation (sunscreens, clothes, etc). 

Particle size measurements were made using an UV-laser, shadow photographic technique. The particle sizing system displayed real-time droplet images onto a television monitor. The images (shadows) were obtained when a pulsed (30 times per second) UV-laser beam was directed through a spray scene onto a synchronized UV-sensitive vidicon camera/recorder. The narrow depth of field used by this system can record shadows from 300 in-focus droplets per second with a resolution down to approximately 0.3 /un in diameter. 

Where There Is Light, There Is Also Shadow Experiments with UV Light... 

Cr patterns are formed on the backside of the glass snbstrate in addition to surface Cr/Au patterns. Cr patterning on both sides enables to define the exposure areas. For finer patterns, exposure areas cannot be defined perfectly dne to the inflnence of UV diffraction at the edge of backside Cr patterns even if Cr shadow layers are formed on both sides of a glass substrate. For this reason the negative resist (CA3000) was formed to avoid formation of Ni walls between metal electrodes. Two additional techniqnes enabled the realization of the 3-D micromesh metal structnres formed on the planar electrodes on the glass surface. 

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