Used for Identification of Human Protozoan Parasites in Molluscan Shellfish

METHODS USED FOR IDENTIFICATION OF HUMAN PROTOZOAN PARASITES IN MOLLUSCAN SHELLFISH 

Methods for identification of human protozoan parasites in the tissue of molluscan shellfish include (1) immunofluorescent antibodies (IFA) alone or in combination with immunomagnetic separation (IMS), (2) polymerase chain reaction (PCR) alone or combined with restricted fragment length polymorphism (RFLP) for genotyping, (3) multiplexed nested PCR, and (4) fluorescence in situ hybridization (FISH). Infectivity of the parasites recovered from the shellfish are usually assessed by mouse bioassays. 

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IV. Methods Used for Identification of Human Protozoan Parasites in Molluscan Shellfish... 

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