Uridine diphosphate glucose UDP-Glc

Phosphorolysis of ribonucleic acid with polynucleotide phosphorylase gives a mixture of the diphosphates of the four common nucleosides, which are transformed into triphosphates with enolpyruvate phosphate and pyruvate kinase. This mixture may be used as such as a source of uridine triphosphate in the preparation of the nucleotide-sugar uridine 5 -(a-D-glucopy-ranosyl diphosphate) ( uridine-diphosphate-glucose, UDP-Glc), or as a... 

Uridine diphosphate glucose (UDP-Glc) serves as a glucosyl donor in many enzymatic glycosylation processes. A multiple enzyme, one-pot, biocatalytic system was developed for the synthesis of UDP-Glc from low cost raw materials maltodextrin and uridine triphosphate. Three enzymes needed for the synthesis of UDP-Glc (maltodextrin phosphorylase, glucose-l-phosphate thymidyly-transferase, and pyrophosphatase) were expressed in Escherichia coli and then immobilized individually on amino-functionalized magnetic nanoparticles. The conditions for biocatalysis were optimized and the immobilized multiple-enzyme biocatalyst could be easily recovered and reused up to five times in repeated syntheses of UDP-Glc. After a simple purification, approximately 630 mg of crystallized UDP-Glc was obtained from 1 L of reaction mixture, with a moderate yield of around 50% (UTP conversion) at very low cost. ... 

Uridine diphosphate glucose may be represented ai UDPG or UDP-Glc the latter form is preferrw if there i the possibility of confusing G for glucose with G for guano sine. 

Figure 11.22 Possible pathways for the synthesis of gangliosides. Cer, ceramide Glc, glucose Gal, galactose GalNAc, N-acetylgalactos-amine CMP, cytidine monophosphate NANA, A/-acetylneuraminic add (sialic acid) UDP, uridine diphosphate. Reproduced from Gurr and James (1980) with permission.

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