Ultra-Small Stationary Phase Particles

These disappointing observations (Butchart 2007) concerning the performance of small particle columns in fast chromatography ( 3 - 4 minute run times) were explained by considerations of the quality of column packing. The degree to which a column is well packed is affected by both the particle diameter and by the column dimensions. The smaller the particle size the greater 

with permission from John Wiley Sons, Ltd. 

---

Section II covers the latest trends in reducing sample preparation time, including direct sample infusion/injection and on-line solid phase extraction (SPE). In Section III, we focus on newer trends in stationary phases and how these phases hope to offer different selectivities compared to current CIS-based phases. Section IV briefly provides a few observations on how new detectors are increasing the versatility of HPLC. Finally, in Section V we examine monolithic columns, small particles packed in short columns, high-temperature LC, ultra high-pressure LC, and parallel injection techniques. 

---