Tyrosinase gene development

Tanaka, S., Yamamoto, H., Takeuchi, S., andTakeuchi, T. (1990). Melanization in albino mice transformed by introducing cloned mouse tyrosinase gene. Development 108 223-227. 

The temporal expression pattern of the mouse tyrosinase gene during embryonic development was not known, and therefore we exploited the... 

Figure 6. Transgene and tyrosinase gene expression in early eye development. Sections are derived from embryonic day 10.5 (A, B), 12.5 (C, D), 13.5 (E), and 17.5 (F) (for details see Beermann etal., 1992a,b). Transgene-specific expression (ptrTyrS) is depicted in A, B, E, F, and tyrosinase gene expression in C and D. In A-D, the same section was photographed both in dark field (A, C) and in bright field (B, D). Bar represents 150 pm in A, B 250 pm in C, D, E and 200 pm in F.

Figure 7. Expression pattern of the mouse tyrosinase gene during embryonic development and its recapitulation in transgenic mice as determined by in situ hybridization (Beermann et al., 1992a). Black box, mouse tyrosinase open box, transgene ptrTyrf striped box, ptrTyr5. Interrupted boxes indicate variations between lines. RPE, retinal pigment epithelium e, days of gestation d0.5, newborn.

Beermann, F., Schmid, E., and Schtitz, G. (1992a). Expression of the mouse tyrosinase gene during embryonic development recapitulation of the temporal regulation in transgenic mice. Proc. Natl. Acad. Sci. USA 89 2809-2813. 

Bennett, D. C., Huszar, D., Laipis, P. J Jaenisch, R., and Jackson, I. J. (1990). Phenotypic rescue of mutant brown melanocytes by a retrovirus carrying a wild-type tyrosinase-related protein gene. Development 110 471-475. 

Porter, S. )., and Meyer, C. J. (1994). A distal tyrosinase upstream element stimulates gene expression in neural-crest-derived melanocytes of transgenic mice position-independent and mosaic expression. Development 120 2103-2111. 

Sequence comparisons show that the CuA-binding sites of M-Hc and the tyrosinases did not develop by gene duplication of CuB, but by the addition of a new copper-binding structure, possibly of a totally new copper protein. In this, M-Hc and most tyrosinases differ from the tyrosinase of Neurospora [34,247],... 

---