Two-dimensional liquid mass mapping

A TWO-DIMENSIONAL LIQUID MASS MAPPING TECHNIQUE FOR BIOMARKER DISCOVERY... 

Wall, D. B. Kachman, M. T. Gong, S. Hinderer, R. Paras, S. Misek, D. E. Hanash, S. M. Lubman, D. M. Isoelectric focusing nonporous RP HPLC A two-dimensional liquid-phase separation method for mapping of cellular proteins with identification using MALDI-TOF mass spectrometry. Anal. Chem. 2000, 72, 1099-1111. 

Buchanan, N. S., Hamler, R. L., Leopold, P. E., Miller, F. R., Lubman, D. M. (2005). Mass mapping of cancer cell lysates using two-dimensional liquid separations, electrospray-time of flight-mass spectrometry, and automated data processing. Electrophoresis 26(1), 248-256. Buick, R. N., Pullano, R., Trent, J. M. (1985). Comparative properties of 5 human ovarian adenocarcinoma celllines. Cancer Res. 45(8), 3668-3676. 

Wang H, Kachman MT, Schwaratz DR, Cho KR, Lubman DM. A protein molecular weight map of ES2 clear cell ovarian carcinoma cells using a two-dimensional liquid separations/mass mapping technique. Electrophoresis 2002 23 3618-81. 

D.M. Lubman, M.T. Kachman, H.X. Wang, S.Y. Gong, F. Yan, R.L. Hamler, K.A. O Neil, K. Zhu, N.S. Buchanan and T.J. Barder, Two-dimensional liquid separation-mass mapping of proteins from human cancer cell lysates. Journal of Chromatography B. 782, 183-196 (2002). 

Instead of polarized noble gases, thermally polarized NMR microimaging was used to study of liquid and gas flow in monolithic catalysts. Two-dimensional spatial maps of flow velocity distributions for acetylene, propane, and butane flowing along the transport channels of shaped monolithic alumina catalysts were obtained at 7 T by NMR, with true in-plane resolution of 400 xm and reasonable detection times. The flow maps reveal the highly nonuniform spatial distribution of shear rates within the monolith channels of square cross-section, the kind of information essential for evaluation and improvement of the efficiency of mass transfer in shaped catalysts. The water flow imaging, for comparison, demonstrates the transformation of a transient flow pattern observed closer to the inflow edge of a monolith into a fully developed one further downstream. 

Haynes, R, I. Miller, R. Aebersold, M. Gemeiner, I. Eberini, M. R. Lovati, C. Manzoni, M. Vignati, and E. Gianazza. 1998. Proteins of rat serum I. Establishing a reference two-dimensional electrophoresis map by immunodetection and microbore high-performance liquid chromatography-electrospray mass spectrometry. Electrophoresis 19 1484-1492. 

S. M. Lubman, D. M. Identification of proteins from two-dimensional gel electrophoresis of human erythroleukemia cells using capillary high performance liquid chromatography/electrospray-ion trap reflectron time-of-flight mass spectrometry with two-dimensional topographic map analysis of in-gel tryptic digest products. Rapid Common. Mass Spectrom. 1999, 73(19), 1907 1916. 

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