Two 1 antibody

Steps in a two 1° antibody different species indirect immunocytochemistry experiment. 

Final Procedure for Two 1° Antibody Same Species with Block-Between. 

Combine Two 1° Antibodies from the Same Species with Block-Bet ween Method... 

A new Experimental Design Chart must be used for this Indirect Immunocytochemistry Block-Between (Table 12.1), because it differs from the chart used previously. Here, the experiment uses cultured cells on coverslips and two 1° antibodies made in mouse, mouse anti-Ag A and mouse anti-Ag B. A new row on the Chart is needed, 4. Block-between, to list two new reagents used to block-between the two 1° antibodies. Normal mouse serum is used at 1 20, which is sufficient to block in most cases. The anti-mouse Fab molecule is used at 20 p.g/ml. Incubations for each of these steps is for 1 h with six rinses after each incubation. Note these blocking incubations must be performed in the order of normal mouse serum first and anti-mouse Fab second. 

Combine Two 1° Antibodies from tbe Same Species with Zenon... 

Multiple 1° antibodies from different species - a method with two 1° antibodies... 

Multiple 1° antibodies made in the same species block between - a method where two 1° antibodies made in same species are used two separate incubations for 1° and 2° antibody separated by a blocking step with normal serum and Fab fragments. 

---