Two step purification of a monoclonal antibody

This example demonstrates the effectiveness of using a high selectivity affinity chromatography technique as a capture step, since only a second gel filtration polishing step was needed to achieve the required level of purity. 

The objective of this work was to produce an efficient, routine procedure for monoclonal antibody purification. 

A more detailed description of this work can be found in Application Note 18-1128-93. 

Salt concentration and pH were adjusted to those of the binding buffer in the capture step. Samples were filtered through a 0.45 pm filter before chromatography. 

Although general standard protocols were supplied with this pre-packed columns, it was decided to further optimise the binding and elution conditions for the specific target molecule. 

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