Tumour inoculation

Many vimses, both DNA and RNA containing, will cause cancer in animals. This so-called oncogenic achvity of a vims can be demonstrated by the observahon of tumour formahon in inoculated experimental animals and by the ability of the vims to transform normal tissue culture cells into cells with malignant characteristics. These transformed cells are easily recognizable as they exhibit such properties as rapid growth and frequent mitosis, or loss of normal cell contact inhibition, so that they pile up on top of each other instead of remaining in a well-organized layer. 

Figure 2.23 Effect of different doses of whole peptidoglycan (WPG) from Bifidobacterium infantis on the growth rate of Meth A tumour. Mice were inoculated subcutaneously with a mixture of 105 Meth A cells and 0 ( ), 10 (A), 20 (A), 25 (O), 50 ( ) or 100(D) M of WPG. (Modified from Tamura et al., 1993.)...

Various celi lines Subdermal tumour in the hamster produced by inoculation of M-I cells, F-53,F-3,F-9,F-24 Undulating tubules, UMS P(F9) [1511... 

The effect of 2-[2-(5-nitro-2-furyl)vinyl]quinoline (LIX) and 20 other compounds on Ehrlich ascites carcinoma EY 33 in mice was tested against Miomycin G in 1964 . Pure strain healthy mice were intraperitoneally inoculated with this tumour. In general the animals will die of the accumulation of tiscites after 10-19 days. Compounds are considered effective when treated mice survive 50 days after the inoculation of the tumour. The compounds found to be effective in this test were the compound LIX), the sodium salt of 2-(5-nitro-2-furyl)vinylquinoline-4-carboxylic acid LXII),... 

In cancer chemotherapeutic studies, it was found that when a single dose of tritiated cytidine is inoculated intraperitoneally into a donor rat, a sufficient amount of the radioisotope is incorporated to result in the heavy positive labelling of more than 99 per cent of the ascite tumour cells. When these isotopically labelled tumour cells are subsequently injected into the blood of recipient animals, the cells can be readily identified in autoradiographs of the lung tissue of these animals [236]. 

Fig. 10.51. Laser-induced fluorescence spectra for an experimental tumour (a human colon adenocarcinoma inoculated in rat muscle) and normal surrounding tissue. The rat had been injected with a dose of hematoporphyrin derivative about two days before the investigation. Two excitation wavelengths, 337 and 405 nm, are chosen to illustrate the influence of the wavelength choice [10.185]...

---