Tumors Nuclear changes

Fig. 1. Time course of cell death parameters in tumor necrosis factor (TNF)-induced apoptosis. Human hepatoma cells (HepG2) were incubated in the presence of 400 nM actinomycin D plus 1.6 ng/ml recombinant human TNF (rhTNF)-a. MTT-dye reduction (parameter of mitochondrial function), lactate dehydrogenase (LDH) release (parameter for cell membrane rupture), and DNA fragmentation (parameter associated with typically apoptotic nuclear changes) were determined over a time period of 24 h. As is characteristic of apoptotic cell death, DNA fragmentation always preceded the other death parameters. Similar findings have been obtained in primary hepatocyte cultures and murine livers...

First cytologic alterations of colon adenocarcinoma cells (Fig. 43) manifested 12 h after administration of I and consisted of nuclear changes (Fig. 44) such as chromatin condensation, enlargement of the nuclear envelope, structural aberrations of the nucleoli and formation of segmented nuclei. Cytoplasmic phenomena occurred 12 h later and manifested by the appearance of lipid droplets and inclusion bodies which often contained cellular debris (Figs. 45, 46). These phenomena indicated cytoplasmic degeneration as well as phagocytotic activity of tumor cells. 

Bilir, A., Altinoz, M.A., Erkan, M., Ozmen, V., and Aydiner, A. (2001). Autophagy and nuclear changes in FM3A breast tumor cells after epirubicin, medroxyprogesterone and tamoxifen treatment in vitro. Pathobiology 69, 120-126. 

C. Thomas, C. Counsell, P. Wood, G.E. Adams, Use of F-19 nuclear-magnetic-resonance spectroscopy and hydralazine for measuring dynamic changes in blood perfusion volume in tumors in mice, J. Natl. Cancer Inst. 84 (1992) 174-180. 

The most frequently observed genetic changes in human tumors affect the gene for a nuclear phosphoprotein of 393 amino acids, which is known as the p53 protein, after its molecular weight. 

EC Activation. EC activation encompasses the active response of ECs to proinflammatory cytokines such as tumor necrosis factor-a (TNF-a), interleukin 1/3 (IL-1/3), or interferon gamma (IFN-7) [15, 16], These responses are characterized as profound morphological and phenotypic changes [15-17], The transcription of the nuclear factor Nf-kB and the... 

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