Tumor biotinylated antibodies

Govindan SV, Griffiths GL, Michel RB, Adrews PM, Goldenberg DM, Mattes MI. Use of galactosylated-streptavidin as a clearing agent with " in-labeled biotinylated antibodies to enhance tumor/non-tumor localization ratios. Cancer Biother Radiopharm 2002 17 307 -316. 

NHS-LC-biotin can be used to add a biotin tag to monoclonal antibodies directed at certain tumor antigens. The biotinylated monoclonals are allowed to bind to the tumor cell surfaces in vivo, and subsequent administration of an avidin or streptavidin conjugate can form the basis for inducing cytotoxic effects or creating traceable complexes for use in imaging techniques (Hnatowich et al., 1987). 

Colloidal gold-labeled (strept)avidin can be used as highly sensitive detection reagents for microscopy techniques (Cubie and Norval, 1989) (Chapter 24). Finally, cytotoxic substances coupled to (strept)avidin can be used to direct cell-killing activity toward a tumor-cell-bound, biotinylated monoclonal antibody (or other targeting molecule) for cancer therapy (Hashimoto et al, 1984) (Chapter 21). 

Paganelli, G., Riva, P., Deleide, G., Clivio, A., Chiolerio, F., Scassellati, G.A., Malcovati, M., and Siccardi, A.G. (1988) In vivo labeling of biotinylated monoclonal antibodies by radioactive avidin A strategy to increase tumor radiolocalization. Int.. Cancer 2, 121-125. 

An avidin-biotin system has been used to attach antibodies in the bilayer of DDSs. Xiao et al. developed a three-step strategy to improve the tumor-to-tissue ratio of anticancer agents [184], Two antibodies specific for the CA-125 antigen that is highly expressed on NIH OVCAR-3 cells were used. These cells were prelabeled with biotinylated anti-CA-125 antibody and fluoroscein isothiocyanate (FITC)-labeled streptavidin (SAv) prior to administration of biotinylated liposomes. Both antibodies were specifically bound to the cell surface of OVCAR-3 cells but not to SK-OV-3 cells, which do not express the specific antibody. Antibody biotinylation did not affect its immunoreactivity. 

Sung CS, van Osdol WW, Saga T, Neumann RD, Dedrick RL, Weinstein JN. Streptavidin distribution in metastatic tumors pretargeted with a biotinylated monoclonal antibody Theoretical and experimental pharmacokinetics. Cancer Res 1994 54 2166-75. 

Paganelli, G., Magnani, P., Belloni, C., Siccardi, A., and Fazio, F. (1992) Two-step tumor targeting in ovarian cancer patients using biotinylated monoclonal antibodies and radioactive streptavidin. Eur. J. Nucl. Med. 19, 322-329. 

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