Tubing centrifuge

Amber-colored bottles Concentration tubes Centrifuge... 

TUBE CENTRIFUGE PLASTIC POLYETHYLENE 118MM LG 18MM OD 15MLCAP12 6640009266966 PG 17.60 ... 

Pipet 25 mL of serum or ascitic fluid into each of four 50-mL polycarbonate tubes. Centrifuge at 10,000g for 30 min at 4°C to remove any large aggregates (see Note 4). 

Gently decant the PBS from the cells and add 10 mL of PBS to the tube and vortex. After suspension is thoroughly mixed, decant to a 15-mL polystyrene centrifuge tube. Centrifuge at 1500g for 10 min to pellet the cells. 

Transfer 1.5 mL of an overnight culture to a microfuge tube. Centrifuge the tube at 4000 X g for 5 minutes. 

Omnimixer (Sorvall) with adapter for 50-ml centrifuge tubes Centrifuge (with a Sorvall SS-34 rotor or equivalent)... 

Each synthesized affinity matrix (50 pL) is mixed with 100 pL of distilled water in an Eppendorf tube, centrifuged for 2 min at 1430g, the supernatant discarded, and 2<- 100 pL regeneration buffer added to the resin. The components are gently mixed and centrifuged for 2 min at 1430g, the supernatant discarded, and 2 100... 

Compact laboratory mixer used for stirring small sample volumes in containers (i.e., test tubes, centrifuge tubes, colorimetric tubes, small flasks). Volume 1(1,3). 

After dialysis is complete, carefully transfer protein solution from dialysis bag into three sterile 50-mL centrifuge tubes. Centrifuge at 34,700 g for 30 min at 4°C. 

Step 5. Transfer the residual solution with precipitate to a 50-mL centrifuge tube. Centrifuge. Discard the supernate. Wash the beaker with 40 mL of 0.1N nitric acid, add the wash to the centrifuge tube with the precipitate, and centrifuge. Discard the supernate. 

Step 7b. Stir precipitate into remaining supernatant solution and decant into 2 centrifuge tubes. Centrifuge and discard supernate. Wash out beaker with 20 ml water, transfer to centrifuge tubes that contain the precipitate, centrifuge, and discard supernate. 

Section 23.), dried and rinsed with distilled water before use. The advantage of these reaction vessels is that many of the reactions can be carried out successively in the same tube. Centrifugation of ethanol precipitates is rapid in the Eppendorf microfuge (12,000 g) and precipitates are collected as barely visible pellets at the bottom of the tube. 

For the cells remove the supernatant and add 500 pL of PBS to the pellet. Resuspend the cells and transfer to a 1.5-mL microcentrifuge tube, centrifuge 20 pL of them, and add 40 pL of SDS-PAGE buffer. Boil and sonicate the sample. Electrophorese 20 pL (equivalent for 1/50 total cells)... 

Add 1 ml of ice cold 70% ethanol to each tube, centrifuge for 5 min at 4°C, and carefully remove the supernatant from the precipitated DNA pellet. This wash is done to remove the salts that were added to the DNA solution to aid in its precipitation, which may affect the activity of the T7 RNA polymerase. 

Alternatively, repeat the experiment but add 20 mg precipitated PbO instead of the concentrated HN03. After the bromine and iodine have been eliminated, add 1 ml water, transfer to a centrifuge tube, centrifuge, and add a few drops of AgN03 solution to the clear centrifugate a white precipitate, soluble in dilute NH3 solution and reprecipitated by dilute HN03, indicates chloride. 

Table VI.19 Identification of Group V cations on the semimicro scale Treat the dry residue (contained in a small crucible) with 1 ml water, stir for 1 minute, and transfer with the aid of a further 05 ml water to a semimicro centrifuge tube. Centrifuge. (1)...

Add cold ether (10-fold volume), mix, and transfer mixture into the centrifuge tube centrifuge. 

Aliquot approx 10 cells in L-15 plus PCS into each of 5 tubes. Centrifuge (2 min at 500g) and discard the supernatant... 

Cells are seeded in a microwell plate 2 to 3 days before the experiment to reach about 80 % confluency on the day of the experiment to favour adherence during subsequent manipulations. Remember that certain cell lines have to be cultured on a special matrix. On the day of the experiment cells in a microwell plate are transferred to a 37°C waterbath. The temperature should be checked with a thermometer since variations may influence exocytosis. Cells are first washed with KRH, than permeabilized with SLO in 0.1 iM free Ca ". The cells are exposed to SLO for 7 min. Subsequently, cells are preincubated (if necessary) at 0.1 xM Ca and then shifted to 0.1 M or 10 jxM Ca ". Finally, the supernatant is transferred to microcentrifuge tubes, centrifuged at 10 000 g for 2 min to sediment any contaminating cells thereafter aliquots are used for determination of the secretory product. 

Resuspend the final washed pellet in 1 mL of PBS and transfer to a 1.5-mL Eppendorf tube. Centrifuge to pellet the cells. 

Extract DNA samples for 5 min with 1.0 mL of chloroform/tube. Centrifuge for 1 min and remove chloroform (lower) layer. Remove the interface with the chloroform. 

Combine cells from triplicate wells of cell culture plates in a fluorescence-activated cell sorting (FACS) tube. Centrifuge, at 300x (1,200 r.p.m. on a Heraeus Megafiige 2.0) for 5 min, remove supernatants carefully and add 1 ml PBS supplemented with 1% FCS (FACS buffer). Centrifuge, again at... 

Preparation of Extracts. Screw-capped culture tubes (16 X 150 mm) with Teflon-lined caps are used for carrying out the extractions. For sera or blank, use tubes containing 9.5 ml of isopropanol. While mixing the contents of the tubes on a Vortex mixer, add 0.5 ml of serum or 0.5 ml of 0.9% NaCl. Cap tubes, centrifuge, and transfer to a clean tube if extracts are to be stored, or sample directly if digestions are to be done at once. 

Trypsinization remove spent medium (20 ml) and wash plate with 20 ml PBS followed by addition of 2 ml Trypsin-EDTA xl solution. Incubate for 5-15 min pipetting to disperse cells. Add 8 ml growth media to stop trypsinization and transfer cell suspension to 15 ml tube. Centrifuge (1,000x5 min) and resuspend cells in serum free media for cell counting. 

Harvest cultured cells by standard procedures and place in 15 mL screw-capped centrifuge tubes. Centrifuge for 5 min at 200g at room temperature. Resuspend the cell pellet at 0.5-1.0 x 106 cells mL in prewarmed 37°C cell-culture medium. Leave cell suspensions in the water bath at 37°C for at least 5... 

Laboratory test tube centrifuges can determine if there is a sufficient density difference between the two phases to consider sedimentation as an alternative. If there is a sharp separation, one can anticipate the same in the field. One can also answer the following questions. Do the solids settle or float Is the solid phase granular or amorphous What is the moisture content The characteristics of the solids indicate the solids discharge design required, i.e., scroll in decanters, or in disk centrifuges, flow-through nozzles or wall valves. 

To the two test tubes in which no precipitate formed in Step 3, add 2 mL of 1 M (NH4)2C03. Precipitates should form in both tubes. Centrifuge, carefully withdraw the supernatant (the liquid above the precipitate), and discard. Add 1 mL of water to each, shake, then add 6 M HC1 dropwise, shaking after each drop, until the solid has dissolved. Then add 1 mL of 1 M Na2S04 solution and shake. A white precipitate forms in the case of the Ba2+ but not in the case of the Ca2+. These observations constitute the positive tests for these ions. The reactions are... 

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