Troubleshooting strategy

C. Some Troubleshooting Strategies ENHANCING HPLC PRECISION... 

This section contains a list of steps the analyst can take to ensure successful HPLC operation and includes highlights of key maintenance and troubleshooting strategies. 

A troubleshooting strategy is given in Table 22.1. It involves five steps. This sequence of steps is shown to parallel the problem-solving strategy of Fo er and LeBlanc discussed in Section 22.1.1. 

Fogler and LeBlanc troubleshooting strategy, 777 Footprints, estimating, 34, 31 Forecasting uncertainty, 326-331 formalin production... 

Not surprisingly, the same strategies and philosophy that govern turboexpander seleetion play a key role in defining maintenanee requirements. Unfortunately, a number of operators never link maintenanee philosophy and troubleshooting to an appropriate extent. They either leave themselves wide open for disastrous repair bills or spend more than they need to on maintenanee. 

The following is a set of guidelines and strategies for troubleshooting HPLC system problems. Further details on HPLC troubleshooting can be found elsewhere. 

Experience with CE method transfer in the biotech/pharmaceutical industry over the past 10—20 years has demonstrated that training is a key element that requires special attention for CE methods. A training video with troubleshooting examples can be very useful. Tips and hints should also be shared during the method transfer process. Other key elements for a successful transfer include selection of the proper testing strategy and assay acceptance criteria. 

The analysis strategy uses LC/MS/MS in combination with a 96-well disk plate SPE. The activities associated with sample preparation and analysis are decoupled to simplify troubleshooting and to allow for greater flexibility. The benefit of this off-line approach is the ability to simultaneously prepare 96 samples with SPE in 1 h, followed by the LC/MS/MS analysis on each sample. Previous approaches with individual SPE cartridges required 2-3 h. 

With its wealth of information and rare focus solely on the subject, this book provides practical tools to help readers better understand these and other problems and then develop optimal solutions and strategies. It discusses mechanisms of organic acid antimicrobial action and facilitates troubleshooting techniques regarding problems with preservatives and microorganisms. It also shows how to predict the effectiveness of a preservation method or application. 

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