Trolox equivalents

Gliszczyhska-Swigl, A. (2006). Antioxidant activity of water soluble vitamins in the TEAC (trolox equivalent antioxidant capacity) and the FRAP (ferric reducing antioxidant power) assays. Food Chemistry, Vol.96, No.l, (May 2006), pp. 131-136, ISSN 0308-8146. 

Regarding antioxidants, the parameter total dietary antioxidant capacity (TDAC) can be taken to reflect antioxidant intake it is defined as the antioxidant capacity of all plant foods and beverages (alcoholic and nonalcoholic) consumed daily in a diet and may represent the amount of antioxidant units (Trolox equivalents) present daily in the human gut (Saura-Calixto and Goni 2006). 

TDAC in the Spanish diet has been estimated at 3,500 pimol Trolox equivalents by the ABTS method. The contribution of each specific food to the TDAC was dependent on both food intake and food antioxidant capacity. The largest contributors to the TDAC were beverages (about 68%) and fruits and vegetables (about 20%). 

Radical Cation 2,2-Azinobis (3-ethylbenzothiazoline-6-sulfonate) (ABTS 1) TEAC Assay (Trolox Equivalent Antioxidant Capacity) or ABTS Assay... 

The Trolox equivalent antioxidant capacity (TEAC) assay was reported first by Miller and others (1993) and Rice-Evans and Miller (1994). They used the peroxidase activity of metmyoglobin to oxidize 2,2 -azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) in the presence of hydrogen peroxide. The TEAC assay is based on the... 

Another assay that is very similar to the ABTS assay is the AGV-dimethyl-p-phenylenediamine (DMPD assay). In the presence of a suitable oxidant solution at an acidic pH, DMPD is converted to a stable and colored DMPD radical cation (DMPD +). Antioxidants capable of transferring a hydrogen atom to the radical cause the decol-orization of the solution, which is spectrophotometrically measured at 505 nm. The reaction is stable, and the endpoint is taken to be the measure of antioxidant efficiency. Antioxidant ability is expressed as Trolox equivalents using a calibration curve plotted with different amounts of Trolox (Fogliano and others 1999). This method is used to measure hydrophilic compounds. The presence of organic acids, especially citric acid, in some extracts may interfere with the DMPD assay, and so this assay should be used with caution in those extracts rich in organic acids (Gil and others 2000). 

In vitro Antioxidant capacity was measured by the method of Trolox equivalent antioxidative capacity (TEAC) [12]. The TEAC assay used 20 pL of the extract sample mixed with a 980 pL solution of ABTS + [2.2 -azobis-(3-ethylenebenzothia-zohne-6-srrlfonic) acid], the decolorization of ABTS ", which is a long-life radical cation and bine-green chromophore, measrrred at 734 nm. Results are expressed as millimoles of Trolox equivalent per kilograms of fresh weight. 

The in vitro antioxidant capacity was measured at 2, 4 and 6 min in duplicate. The results presented in Fig. 41.3 are obtained at 4 min (the best correlation coefficient for A Abs calculation, data not shown) and Trolox equivalents were calculated... 

Sample Total phenolics (mg CE /100 mg) Antioxidant activity (pM Trolox equivalents/100 mg)... 

Trolox Equivalent Antioxidant Capacity (TEAC) assay... 

The area under curve for the sample (AUCsaj pie), reduced by the area under curve plotted for the blank sample (AUCbianJ is referred to as "net AUC". Moreover, the net AUC is calculated for a series of dilutions of Trolox and a calibration curve is plotted, showing the relationship between net AUC and the concentration of Trolox. The results of the assay which refer the net AUC of the sample to the calibration curve are expressed as Trolox equivalent. 

Trolox equivalent capacity (TEAC) assay is based on antioxidants capability to reduce radical cations 2.2-azinobis(3-etilbenzotiazoline 6-sulfonat) (ABTS) and thus to inhibit absorption in the long wave part of the spectra (600 nm). Water-soluble vitamin E derivation Trolox [42] is used as a standard. 

TRAP, Total peroxyl-radical-trapping antioxidant capability of plasma TEAC, Trolox equivalent antioxidant capacity FRAP, ferric-reducing activity of plasma ORAC, oxygen-radical-absorbing capacity PCA, perchloric acid-soluble fraction ABAP, 2,2 -azobis(2-amidoproane). 

Material (anticoagulant) Value (Trolox equivalents) Method Ref. 

Red wines have higher TAC than white wines. Red Chilean wines were found to have TAC of 25.1-33.3 mM, whereas TAC of white wines was 2.9-52 mM Trolox equivalents (C3). Other studies found TAC of 12-14 mM for Californian Pinot Noir, Rioja, and Bouzy Rouge, 16 mM for Australian Shiraz, and 23 mM... 

Milligrams of chlorogenic acid equivalents per gram of dry weight. Micromoles of Trolox equivalents per gram of dry weight. 

TEAC Trolox equivalent antioxidant Rice-Evans447... 

ABTS assay, phycoerythrin assay TEAC (mM Trolox equivalent to 1-mM test substance)... 

Phycoerythrin assay OFtAC, oxygen radical absorbance capacity pmol of Trolox equivalents... 

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