Tris- amino-methane

Hydrazinoyl chlorides react with ketene aminals in the presence of triethylamine to give diaminopy-razolines (544 equation 243). From tetraaminoethylenes and amines or amine derivatives or heterocu-mulenes tris(amino)methane derivatives, e.g. (545) and (546) (Scheme 101) are formed. Reactions of this type have been reviewed. ... 

Bis-(2-hydroxyethyl)amino-tris-(hydroxymethyl)methane (BIS-TRIS) [6976-37-0] M 209.2, m 89 , pK 6.46. Crystd from hot 1-butanol. Dried in a vacuum at 25°. 

TRIS (HYDROXYMETHYL) AMINO-METHANE HYDROCHLORIC ACID 8.07 6.8 to 8.5... 

Primary standard tris-(hydroxymethyl)amino methane, also known as THAM or TRIS (FW = 121.14 g/mol), is used to standardize a hydrochloric acid solution. If 0.4922 g of THAM is used and 23.45 mL of HC1 is needed, what is the normality of HC1 ... 

For standardizing acid solutions, primary standard tris-(hydroxymethyl)amino methane, THAM (also referred to as TRIS), can be used. Its formula is... 

A variety of buffers is used in electrophoresis. The selected buffer must contain ions to carry the current. Other than current-carrying capacity, the most critical criterion for buffer selection is the stability of the sample to be analyzed. Many proteins are unstable in acidic pHs, so alkaline buffers are frequently employed. Tris-(hydroxymethyl)amino methane (TRIS or THAM), sodium acetate, and ethylenedi-aminetetraacetate (EDTA) are common solutes in buffers, with pHs between 7.9 and 8.9 typical. (Refer to Chapter 5 for a discussion of buffers.) These buffers also work well with nucleic acid fragments. In addition, phosphate buffers, e.g., 10 mMK3P04, are often used with nucleic acid fragments (1.0 mM = 0.0010 M). 

Prepare 100 mL (500 mL if the gel is to be run under the buffer) of an electrophoresis buffer that is 20 mM tris-(hydroxymethyl)amino methane (TRIS or THAM), 6 mM sodium acetate, and 1 mM disodium EDTA. Adjust the pH of this solution to 7.9 using concentrated HC1. Also prepare small volumes of solutions of hemoglobin and cytochrome C in the buffer (the concentration is not important) and also a mixture solution of these two solutes. Add a quantity of sucrose to each. 

Prepare a buffer solution that is 20 mM tris-(hydroxymethyl)amino methane (TRIS or THAM), 6 mM sodium acetate, and 1 mM disodium EDTA. Adjust to pH = 7.9. 

THAM, or TRIS, is tris-(hydroxymethyl)amino methane. Its structure is (HOCH2)3CNH2. It is a base because it contains the -NH2 group, which accepts a hydrogen ion to form -NH3+. It is useful as a primary standard because it possesses all the qualities sought in a primary standard, as discussed in Section 4.6.2. 

The sodium acetate-acetic acid combination is one of the most widely used buffers, and is usually referred to simply as acetate buffer. Other buffer combinations commonly employed in chemistry and biochemistry include carbonate-bicarbonate (sodium carbonate-sodium hydrogen carbonate), citrate (citric acid-trisodium citrate), phosphate (sodium dihydrogen phosphate-disodium hydrogen phosphate), and tris [tris(hydroxymethyl)amino-methane-HCl]. 

Glycinamide hydrochloride Tris (tris(hydroxymethyl)-amino methan 2-amino-2-hydroxy methyl-propane-1.3-diol Trizma)... 

Tris(hydroxymethyl)amino-methane (tris or tham)... 

Tri s (hydroxymethyl) Amino] -Methane Trinitrate (or 2-Amino-2-hydroxymethyl-l,3-pro-panediol trinitrate). 

Ami no-2-hydroxymethyl-1,3-propanediol Trinitrate. See Tris(hydroxymethyl)-amino-methane Trinitrate... 

Fig. 23 Pseudo-first-order rate constants for the hydrolysis of NPAlk (n = 2-16) in the absence and in the presence of 1 as a function of alkanoate chain length n, catalyst concentration, and buffer system circles 7.5 x 10-5 molL-1 1 in Tris(hydroxymethyl)amino-methane (7ns) buffer solution closed up triangles 2.5 x 10-5 molL-1 1 in Tris buffer solution closed down triangles 2.5 x 10-5 molL-1 1 in phosphate buffer solution open squares 2.5 x 10-5 molL-1 1 in borate buffer solution open down triangles in Tris buffer solution only closed squares in phosphate buffer solution only open up triangles in borate buffer solution only. (Reprinted with permission from [73]. Copyright 1996 American Chemical Society)...

Q = 0.2 M tris acid maleate (24.2 g of tris (hydroxymethyl) amino methane + 23.2 g of maleic... 

Similar to indirect absorbance detection, indirect fluorescence has been employed to detect MPA, EMPA, IMPA, and PMPA, using tetrakis(4-sul fopheny 1 iporphine (TSPP) as the indirect probe (19). A violet diode laser operating at 415nm was used for excitation. Using an electrolyte composed of 50 tM TSPP and 5mM [Bios(2-hydroxyethyl)-amino]tris-(hydroxymethyl)methane (Bistris) at pH 7.2 under normal polarity, baseline separation was achieved in less than 2 min. A limit of detection of 0.1 xM (9ppb) for MPA was achieved. 

Selenium-enriched yeast, regarded as one of the most interesting materials for Se speciation, has also been studied through extraction procedures with water and buffered solutions as well, typically with 30 mmol l-1 tris(hydroxymethyl)amino-methane (Tris)-HCl at pH = 7.0. The investigations performed by several independent research groups almost always led to the same results both water extraction (at 50-90°C for l-2h) and Tris-buffered extraction (at 37°C for 1 h) achieved about 10 percent extraction of Se, the main Se species identified being Se-adenosyl-homocysteine, a small amount of free SeMet and some nonmetabolized, inorganic Se(IV) in the fermentation-media [21, 40, 41, 43, 44]. 

Tris Buffer Solution Dissolve 6055 g of Tris(Hydroxyl-methyl) amino methane and 0.147 g of calcium chloride dihydrate in 1 L of water. Adjust the pH to 7.0 with 1M hydrochloric acid. 

Crownpak CR(+). Chiral separation of gemifloxacin was performed in analytical counter-current chromatography using (- -)-(18-crown-6)-tetra-carboxylic acid as CSP. A successful separation of gemifloxacin enantiomers could be achieved using a two-phase solvent system composed of 1-butanol-ethyl-acetate-bis(2-hydroxyethyl)amino tris (hydroxymethyl)methane acetate buffer with a small amount of CgH4 [19]. 

Complementary dendritic hexamers based on a central scaffold made up of linked pentaerythritol and tri(hydroxymethyl)amino methane units have been found to also exhibit liquid-crystalline properties. This star-shaped scaffold was used to create supermolecules containing two different hemispheres, referred to thereafter as Janus supermolecular Hquid crystals (Fig. 76) [323,324]. One of the hemisphere contains three cyanobiphenyl end-groups, whereas the other lobe consists of three chiral phenyl benzoate mesogenic moieties laterally attached. The type of mesophase observed (N ... 

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