Triphosphopyridine nucleotide, reduced TPNH

SC Secretory component TPNH Triphosphopyridine nucleotide (reduced)... 

In accordance with the recommendations of the commission on enzymes of the International Union of Biochemistry (see Nature 188, 464-466, I960 Science 182, 1548-1550, 1960 and Report of the Commission on Enzymes of the International Union of Biochemistry 1961, I.U.B. Symposium Series, Vol. 20, Pergamon Press, London) nicotinamide-adenine dinucleotide (NAD) has been substituted fordiphosphopyridine nucleotide (DPN) nicotinamide-adenine dinuoleotide, reduced form (NADHj), for diphosphopyridiiie nucleotide, reduced form (DPNH) nicotinamide-adenine diuu-cleotide phosphate (NADP), for triphosphopyridine nucleotide (TPN) and nicotinamide-adenine dinucleotide phosphate, reduced form (NADPH2), for triphosphopyridine nucleotide, reduced form (TPNH). 

The reduction of 5-dehydroshikimate to shikimate by partially purified 6-dehydroshikimate reductase has also been studied. A cofactor requirement for reduced triphosphopyridine nucleotide (TPNH) was observed, and the equilibrium constant, at neutral pH, for the reaction... 

TPN and TPNH = triphosphopyridine nucleotide and its reduced form, respectively. 

The enzymic hydroxylation of kynurenine has been reported by several groups of workers (62,63). The enzyme is present in rat liver mitochondria and can be solubilized by sonic oscillation or digitonin treatment (63a). Reduced triphosphopyridine nucleotide (TPNH) is cifically required for the hydroxylation and there is a stoichiometric relationship between the TPNH oxidized and the hydro kynureiiine formed (62). Molecularo gen is also required for the hydroxylation, as indicated by 0 studies ( ). The... 

The cofactor requirement of lljS-hydroxylase was clarified by the discovery that reduced triphosphopyridine nucleotide (TPNH) is essential and specific and that the role of citric acid cycle components and the other cofactors in the system appears to be that of supplying endogenously formed TPNH (288,723) by the sequence of reactions shown in equations (98) to (100). TPNH is oxidized in the absence... 

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