Trimethylsilyl ethers of methylated

Trimethylsilyl ethers of methyl glycosides have been used in analyzing bacterial endotoxins that contain 3,6-dideoxyhexoses, and, because of the volatility of such glycosides, careful temperature-programming is essential.373 Methyl D-glucosides and analogs have been... 

Tables XX (see p. 80) and XXI (see p. 82) record examples of separations that used trimethylsilyl ethers and acetates of methyl 0-methylgIycosides. Tables of retention times of trimethylsilyl ethers of methyl mannosides and of acetates of methyl glucosides are available. ...

Retention times given relative to the bis(trimethylsilyl) ether of methyl deoxycholate. 

In a recent study Hofmann et al. (116) studied the bile acid composition of bile from germ-free rabbits. Deproteinized bile was first analyzed by thin-layer chromatography for a tentative identification of the conjugated bile acids. After alkaline hydrolysis and methylation, bile acid methyl esters were analyzed by thin-layer and gas chromatography. Trifluoroacetate esters and trimethylsilyl ethers of methyl cholanoates were run on QF-1 or Hi-Eff-8B columns, respectively. Gas chromatography-mass spectrometry was used for the final identifications. 

Fig. 5. Mass spectra of methyl 3a,12a-dihydroxy-5/3-cholanoate (1) and its trifluoroacetate (2) and trimethylsilyl ether (3) and of the trimethylsilyl ethers of methyl 3a,12a> (4) and 3, 12o >dihydroxy>5a -cholanoates (5).

Fig. 7. Mass spectra of the trimethylsilyl ethers of methyl 3a,7a,12a-trihydroxy-5a- (1), 3a,7a,12a-trihydroxy-5/3- (2), and 3a J8 12a-tnhydroxy-5iS-cholanoates (3).

---