Triethylaminoethyl cellulose powder Serva

Venom of the Malayan pit-viper Agkistrodon rhodostoma Triethylaminoethyl cellulose powder (Serva) tris(Hydroxymethyl)aminomethane... 

Triethylaminoethyl cellulose powder (Serva) of capacity 0.71 m.equiv./gm is suspended in 2 M sodium chloride buffered with 0.1 M tris/phosphate pH 6.0 and the slurry is packed into a glass column 3.6 cm in diameter until the height of the packed material reaches 20 cm. The column is washed with a further 2 L of the solvent used for preparing the slurry and is then equilibrated with 0.01 M tris/phosphate buffer pH 8.5. Tris is an abbreviation for tris(hydroxymethyl)aminomethane. 330-360 mg of crude A. rhodostoma venom is dissolved in 20 ml of 0.01 M tris/phosphate buffer pH 8.5, centrifuged to remove insoluble material, and the clear supernatant is applied to the column. The fractionation is carried out at room temperature at a flow rate of 90-100 ml (35 ml/hour). The protein concentration in the eluate is estimated from the extinction of the solution at 280 m/t in 1 cm cells. The chromatogram is developed with the following buffers. In all cases the molarity of the buffers are with respect 40 to tris. 

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