Serva

Resolution of Optical Isomers), Gakkai Shuppan Senta, Tokyo, Japan, 1989, Chapt. 11, pp. 132—143 Chiral HPLC columns are available from Regis, Spelco, and AST in the United States Merck, Nagel, and Serva in Germany LKB in Sweden and Daisem, Sumitomo, and Toso in Japan. 

Holz B, Klimasauskas S, Serva S, Weinhold E (1998) Nucleic Acids Res 26 1076... 

Materials required Sample solution in methanol, distilled water, 20% Na2C03, Folin-Ciocalteu s phenol reagent, water bath, graduated tube, cuvette, micro pipette (0.1 ml), pipette of 1,2 and 10 ml, test tubes of 20 ml, pure ferulic acid (Serva, Germany), Shimadzu UV 160 spectrophotometer. 

Procedure A 0.002 ml aliquot of the sample solution in methanol was taken and 7 ml distilled water plus 0.1 ml Folin-Ciocalteu s phenol reagent was added and after 3 min 0.2 ml of 20% Na2C03 was included. After boiling at 90 °C (exactly 5 min) samples were cooled at room temperature and were diluted with HzO to 10 ml volume. Only distilled water and reagents were used as a blank. The absorbance of total phenolics was measured at 660 nm spectrophotometrically (a Shimadzu UV 160 spectrophotometer) as per Feldman and Hanks (1968), with a sensitivity of 0.05 pig/g d.w. A standard curve was constructed with different concentrations of ferulic acid (Serva, Germany). Concentrations of ferulic acid varied from 0.33-80jig/ml (Table 1). 

Investigations of the effects of UV- and hypochlorite-induced oxidative modification of 20 amino acids and human serum albumin (HSA) on their antiradical properties showed unexpected results [36], Seven amino acids (cystine, histidine, methionine, phenylalanine, serine, tryptophan, and tyrosine) and HSA developed ACW following oxidation (see examples in Fig. 14). The fresh (produced in 1998) HSA from Serva had no antiradical capacity, but it acquired this quality during irradiation. The out-of-date HSA sample (Dessau, GDR, 1987, expiration date 7/1/1992) showed a remarkable ACW even in an unirradiated state. 

Ci2H25S0itNa (NaDDS) from B.D.H. or Serva (Germany) Na-deoxy-cholate (NaDOC) from Sigma Cetyl trimethylammonium bromide (CTAB) from Schuchardt, were used as supplied by the respective manufacturer (over 99 % purity). Distilled water was used after treatment with the Millipore Q filter. 

Coupled GC-EAD detection analyses of pheromone gland extracts revealed one EAD active compound produced by female Lymentra lucescens and by female L. serva (Lepidoptera Lymantriidae). This compound was identified as 2-methyl-(Z )-7-octadecene by the usual analytical techniques and by comparison with an authentic synthetic compound. 

Vdkaitis, G., Merkiene, E., Serva, S., Weinhold, E. and Klimasauskas, S. (2001) The mechanism of DNA cytosine-5 methylation. Kinetic and mutational dissection of Hhai methyltransferase. The Journal of Biological Chemistry,... 

In the samples 5 and 6 the source of Si and Al is different and respectively Silica Aerosil (Serva) and Aluminium hydroxide (Serva)... 

Serva Chiral BDcx = SilOOPolyol Cavity p-Cydadextrin... 

Chiralpak, Daicel Tokyo similar columns available from Serva, Heidelberg Machcrcy Nagel, Diiren 1992. 

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