Triethylamine phosphate

A solution of bis-triethylamine phosphate was prepared by slowly adding 2.36 ml of B5% phosphoric acid to 20 ml of acetonitrile containing 9.9 ml of triethylamine at 20°C. This solution was added to a stirred solution of 4.70 g of 9a-fluoro-11(3,170,21 -trihydroxy-160-methyl-1,4-pregnadiene-3,20-dione 21 -methanesulfonate and 20 ml of acetonitrile. The mixture was heated under reflux for four hours and then evaporated under reduced pressure to a volume of 12 ml. This mixture was a concentrated solution of 9a-fluoro-11(3,170,21 -tri-hydroxy-160-methyl-1,4-pregnadiene-3,20-dione 21 -phosphate triethylamine salt with some inorganic phosphate. 

The steroids aldosterone, cortisone, cortisol, 11-P-hydroxyandrostenedione, corticosterone, and rostenedione, 11-desoxycorticosterone, 17-hydroxy-progesterone, and progesterone have been performed on Ultrasphere ODS using methanokwater.19 Ranitidine N-[2-[[[5-[(dimethylamino)methyl]-2-furanyl]-methyl]thio]ethyl]-N1-methyl-2-nitro-l,l-ethenediamine has been separated using a p-Bondapak C18 column operated with acetoni-trile methanol water buffered with triethylamine phosphate.117 Pyridoxal-5 -phosphate and other B6 vitamers, including pyridoxamine phosphate, pyri-doxal, pyridoxine, and 4-pyridoxic acid, were separated as bisulfite adducts... 

Medina and Phillips (36) analyzed the peptide pattern obtained by the enzymatic hydrolysis of proteins isolated from beef, pork, chicken, and soybean sequentially, using TLC and HPLC to identify the proteins. The analysis was performed using RP-HPLC on a /zBondapak C8 column under isocratic conditions using triethylamine phosphate buffer (0.0833 M, pH 3) as solvent. Figure 5 depicts the peptide pattern of fraction IV isolated from beef, soybean, chicken, and pork by TLC. Identification of the different foods was accomplished by applying discriminant analysis to the peptide pattern. 

D-enantiomers of amino acids have been frequently reported in various tissues of diverse organisms. A simple and rapid method of separating optical isomers of amino acids on a reverse-phase thin layer chromatography plate is described. Amino acids, derivatized with l-fluoro-2,4-dinitrophenyl-5-L-alanine amide, were spotted onto a reverse-phase thin layer chromatography plate. Acetonitrile in triethylamine-phosphate buffer was used as the developer. 

Figure 4.15. Reversed-phase HPLC of a mixture of peptides produced by digestion of cytochrome C with the protease trypsin. Higher resolution (eight major peaks) and shorter retention times are obtained using hydrochloric acid in the mobile phase buffer compared with using triethylamine phosphate (seven major peaks, with more overlapping) (PerSeptive...

Sample preparation Weigh out powdered sample containing 51 mg acetaminophen, add 80 mlj MeOH, sonicate for 10 min, dilute to 100 mL with MeOH, centrifuge. Remove a 5 mL aliquot of the supernatant and add it to 1 mL 2 mg/mL resorcinol, add 2 mL MeOH, make up to 20 mL with 50 mM pH 3.0 triethylamine phosphate, inject an aliquot. 

Mobile phase THF 50 mM pH 3.0 triethylamine phosphate 12 88 Flowrate 0.6 Injection volume 20... 

Mianserin Plasma LPME Triethylamine-phosphate, pH 5.0, 2mmoir HP- 3-CD Patient samples... 

Ofloxacin and metabolites Urine Direct injection Triethylamine-phosphate, pH 2.0, 0.3mmol SB-jS-CD Urinary excretion, stereoselective metabolism... 

Mobile phase A filtered and degassed mixture of triethylamine phosphate solution, [1] tetrahydrofuran, and acetonitrile (14 5 1). Chromatographic system A liquid chromatograph equipped with a 210 nm detector and a 4.6 mm x 25 cm Cjg column. 

In reversed-phase chromatography AR for the three different derivatives were 0.02-0.09, 0.03-0.10, and 0.14—0.55, respectively, with acetonitrile (30-50%) in triethylamine phosphate buffer, pH = 5.5, as eluent. Resolution was always better for diastereosomers obtained with FDNP-L-Val-NH2 under normal- and reversed-phase conditions. Derivatives appeared as bright yellow spots. 

Aspartame and saccharin were quantitated in dietary formulations by separation on a Ci8 column (A = 210nm for aspartame and 270nm for saccharin). Separation from other matrix interferents (e.g., acesulfame, 4-sulfamoylbenzoic acid, resorcinol (internal standard), p- and o-toluenesulfonamide) was done using a 5/5/90 THF/methanol/water (80 mM triethylamine phosphate buffer at pH 3.0) in <20 min [847]. Excellent resolution and peaks shaped were observed. Standards were in the 5-lOOp.g/mL range. It should be remembered that both methanol and THF have significant absorbance at 210 nm but are used effectively in this case because they are present at very low levels. 

Eight heterocyclic aromatic amine carcinogens were extracted from cooked meats. A Cjg column and a complex 30-min 95/5- 45/55 water (lOmM triethylamine phosphate at pH 3.6)/acetonitrile resolved 2-amino-1-methyl-, 2-amino-3,8-dimethyl-, and 2-amino-3,4,8-trimethylimidazo[4,5-/]quinoxaline with monitoring by UV (A = 262 nm) and fluorescence (A = 307 nm, ex 370 nm, em). A 40-min 95/5—>-20/80 water (25 mM triethylamine phosphate at pH 3.6)/acetonitriIe gradient on the same column resolved 2-amino-1,6-dimethylimi-... 

Dicarboxylic acids (Cg-Cig, even) were analyzed as their mono-coenzyme A, mono-camitine, and 4-nitrobenzyl esters [1063]. The mono-coenzyme A esters were baseline resolved on a C g column (photodiode array detector, A = 200-300 nm) using a complex 45-min 5/95 - 50/50 acetonitrile/water (50 mM KH2PO4 buffer at pH 5.3) gradient. Peak shsqjes were excellent. The mono-camitine esters were analyzed as their 4-bromophenacyl derivatives on a Cg column (A = 260 nm) using a complex 40-min 60/38/2 -> 95/0/5 acetonitrile/water/water (0.15 M triethylamine phosphate buffer at pH 5.6) gradient. The 4-nitrobenzyl derivatives were resolved on... 

Marfey [35] resolved l-fluoro-2,4-dinitro-5-L-alanine amide (FDAA) derivatives of DL-amino acids through HPLC, using a reversed-phase Cis stationary phase and a linear gradient of mobile phase, from 10 to 40% of acetonitrile in triethylamine-phosphate buffer (pH 3.5). 

Mobile phase acetonitrile-triethylamine-phosphate buffer (50 mM, pH 5.5). Time 20 min temperature 25°C solvent front 75 mm. 

Mobile phase acetonitrile with triethylamine-phosphate buffer (50 vaM, pH 5.5, v/v). Solvent front 9 cm. 

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