Tri saccharoses

Tri-saccharoses or Hexo-trioses.—(2) The other less important group of the poly-saccharoses that are true sugars is that of the tri-saccharosesy or hexo-trioses. These split, as their name indicates, into three molecules of hexose mono-saccharoses. The formula corresponds to the composition C18H32O16. The hydrolysis may be represented by the reaction,... 

Only one tri-saccharose is important. It is known as raffinose and has the composition Ci8H320]6. It is found in beets and is present in the molasses after the sucrose sugar is crystallized out. It is also found in barley and in cotton seeds. When this tri-saccharose hydrolyzes it yields first a di-saccharose known as melibiose and a monosaccharose fructose. The di-saccharose is then further hydrolyzed and yields two molecules of mono-saccharose, viz., glucose and galactose. The complete hydrolysis of the tri-saccharose, therefore, is as follows. 

From Formaldehyde.—Historically and physiologically, the most important synthesis of hexose mono-saccharoses is from formaldehyde. In 1861 Butlerow found that dioxymethylene (tri-oxymethylene), produced by polymerizing formaldehyde, yielded with hot lime water a sweet substance to which he gave the name of methylenitan. The substance reduced Fehling s solution, but was optically inactive and non-fermentable with yeast zymase. Later, Loew obtained a sweet, non-fermentable syrup by the direct action of lime-water on formaldehyde. This substance he called formose. He afterward obtained what he considered another sugar by the action of magnesium hydroxide upon formaldehyde. This substance was fermentable by yeast and to it he gave the name of methose. In 1887, Fischer and Tafel... 

Synonyms a-D-Glucopyranoside, 0-acetyl-tris-0-(2-methyl-1 -oxopropyl)-P-D-fructofuranosyl, acetate tris (2-methyl propanoate) Saccharose acetate isobutyrate SAIB Sucrose acetoisobutyrate Sucrose, diacetate hexaisobutyrate Classification Sucrose derivative... 

Reagents methanol, chloroform (Merck, Germany), saccharose, Tris, FCCP (carbonylcyanide-/>-trifluoromethox5q)henylhydrazone), malate, glutamate, succinate (Sigma, USA, BSA (Sigma, USA), HEPES (MP Biomedicals, Germany). 

In May-June, the growing parts of youg pine needles (10g) are ground in 180 ml of Tris-HCI 100 mM, pH 8.3, 0.15 M saccharose, 2 mM p-mercaptoethanol, soluble PVP 1%. After centrifugation, lOO.OOOg supernatants are frozen at -50°C for subsequent cell-free assays and purification procedure. All the above steps and all subsequent operations were carried out at 4°C. 

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