Translation initiation site detecting

The biological significance of the existence of two initiation sites for translation of poliovirus RNA In vitro depends upon whether or not these sites are utilized for translation vivo. Although all of the identified translational products do appear to be made in equal molar amounts, when corrected for alternate cleavage pathways and protein degradation (45 "47> said see chapter 7 of this book), the relative synthesis of Wg (which has not been detected free of RNA in the cytoplasmic extracts of infected cells), has not... 

The tasks of transcriptional and translational signal recognition involve the prediction of promoters and sites that function in the initiation and termination of transcription and translation. Bacterial promoter sites, specifically the Escherichia coli RNA polymerase promoter site, are now very well characterized. The main problem is that the two conserved regions of the bacterial promoter, the -10 and -35 regions, are separated from each other by 15 to 21 bases, making the detection of the entire promoter as a single pattern difficult. Eukaryotic promoters are less well characterized than their bacterial equivalents. The major elements are the CCAAT box, GC box, TATA box and cap site. 

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