Transfer activity coefficient comparative estimates

Taking the active pulse height as 0.05 m and the pulse velocity as 1 m/s, we derive for the mass transfer coefficient in the gas-continuous zone, 11, a value of 10 m/s and in the pulse proper, k, a value of 6 10 m/s. These values compare very well with those given in literature (5, 6) for both gas-continuous and dispersed bubble flow regimes. An estimate of k can also be made by means of the penetration theory, taking the respective liquid in and outside the pulse as the basic for the calculation of the con-... 

Determination of Alkaline Phosphatase Activity. Vomeronasal and olfactory epithelia were removed and transferred to 0.9% NaCl solution where they were maintained at 4°C. All soluble forms of enzymes were washed out from the surface of the receptor tissue (see Chukhrai et al., 1992 for details). Using procedures that are described in detail in Chukhrai et al. (1992), alkaline phosphatase activity in olfactory and vomeronasal epithelia was determined at pH=8.3 in 0.9% bicarbonate buffer. Disodium p-ni-trophenol phosphate (Sigma) was used as a substrate its initial concentration was 8.1 x 10" M in the buffer. The increase of p-nitrophenol (the product of p-nitrophenylphosphate hydrolysis) was measured with a double-beam spectrophotometer at 400 nm every 30 sec. The velocity of the reaction was determined by estimating the angle of the slope (optical units [OU]/min). The obtained values were divided into the extinction coefficient (C ) under corresponding pH values to obtain the reaction velocity values in pM/min. Effective parameters of the Michaelis-Menten equation were compared using standard procedures (Chukhrai et al., 1992). 

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