Transcription uncoupling

Kocgozlu L, Lavalle P, Koenig G et al (2010) Selective and uncoupled role of substrate elasticity in the regulation of replication and transcription in epithelial cells. J Cell Sci 123 29-39... 

A similar approach was followed for displaying a random library of 20-mers using a wheat germ uncoupled transcription and translation system (Gersuk et al., 1997), and several peptides were isolated that bound to prostate-specific antigen, but not to bovine serum albumin. No affinities were reported. 

The molecular mechanism of the NO pro-inflammatory activity is also multifaceted NO regulates the inflammatory responses by cell-specific inhibition of the transcription factor NF-kB, IL-ip, interferon-y (IFNy). At sites of inflammation, increased free radical activity is associated with the activation of the neutrophil NADPH-oxidase and/or the uncoupling of a variety of redox systems, leading to a substantial increase in ROS. Free radicals thus produced, have the capacity to mediate tissue destruction, either alone or in concert with proteases [53]. 

M. E. Young, S. Patil, J. Ying, C. Depre, H. S. Ahuja, G. L. Shipley, S. M. Stepkowski, P. J. Davies and H. Taegtmeyer, Uncoupling protein 3 transcription is regulated by peroxisome proliferator-activated receptor (alpha) in the adult rodent heart, FASEB J15, 833-845 (2001). 

IX. POTENTIAL UNCOUPLING OF TRANSCRIPTION AND TRANSLATION IN THE ONE-CELL EMBRYO... 

Additional experiments that assess expression of a luciferase reporter gene at both the protein and mRNA levels also suggested an uncoupling of transcription from translation (Nothias et al., 1996). Injection of the reporter gene into the nucleus of a two-cell blastomere was followed by the rapid expression of both luciferase mRNA, as detected by a quantitative RT-PCR assay, and luciferase activity. In contrast, analysis of S-phase-arrested, one-cell embryos revealed that, while luciferase mRNA was readily detected at a time that corresponded to G2 of the one-... 

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