Trans-membrane domains

The pecM gene encodes a protein of 297 amino acids with a calculated molecular mass of 32 kDa. The predicted PecM protein displays the characteristics of an integral membrane protein since it is largely hydrophobic, with potential trans-membrane domains. SubceUular firactionation confirmed that PecM is anchored into the bacterial inner membrane whereas PecS is... 

In the CNS of mammals, two proteins are needed to accomplish the dual role of adhesion at both myelin bilayer surfaces that P0 effects in the PNS. The four-trans-membrane-domain proteolipid protein (PLP) is likely to be responsible for adhesion of the extracellular surfaces, while the very basic cytoplasmic myelin basic protein... 

Fig. 5.1 Schematic drawing of membrane association modes of peptides A Integral membrane proteins (1) major fd coat protein gpVIII of bacteriophage Ml 3 (pdb lfdm), anchored by an 18-residue trans-membrane hydrophobic helix (2) bovine rhodopsin, a 7 trans-membrane domain (G-protein-coupled) receptor (pdb lf88) (3) ion channel peptaibol Chrysospermin C (pdb lee7), and B Peripheral membrane proteins (1) neuro-...

Hu J, Asbury T, Achuthan S, Li C, Bertram R, Quine JR, Fu R, Cross TA. Backbone structure of the amantadine-blocked trans-membrane domain M2 proton channel from Influenza A virus. Biophys. J. 2007 92 4335-4343. 

Park SH, Mrse AA, Nevzorov AA, Mesleh MF, Oblatt-Montal M, Montal M, Opella SJ. Three-dimensional structure of the channel-forming trans-membrane domain of virus protein u (Vpu) from HIV-L J. Mol. Biol. 2003 333 409-424. 

Sliiang R, Thompson LM, Zhu YZ, Church DM, Fielder TJ, Bocian M, et al. Mutations in the trans-membrane domain of FGFR3 cause the most common genetic form of dwarfism, achondroplasia. Cell 1994 78 335-42. 

The sarcoplasmic reticulum Ca -ATPase (SR Ca -ATPase) was first purified by MacLennan in 1970. Presently it is the best characterized Ca -ATPase. A schematic model and a summary of some properties are given in Figure 3.11." Ten hydrophobic segments of about 20 amino-acid residues each are revealed by hydropathy plots, and these segments are assumed to span the membrane as a-helices. (For the one-letter codes for amino acids, see Appendix B in Section IX.) The phosphorylation site has been identified as Asp-351, and the nucleotide binding domain is following the phosphorylation domain. The Ca -binding sites are located within the predicted trans-membrane domains... 

A Ca -binding domain is found in the cytosolic region adjacent to the trans-membrane domain. Post-translational modifications to the scramblase that alter activity include acylation and phosphorylation. There is currently much interest in the regulation of scramblase function as it plays a critical role in the extemalization of PS, a process... 

A six-trans-membrane domain, nonselective cation channel, the vanilloid receptor of type 1 (VRI or TRPV1) (Caterina et al., 1997) is the first and only member of the family of the TRP channels discovered so far to be activated by capsaicin, the pungent component of hot chili peppers (Holzer,... 

Attempts have been made to model G protein-coupled receptors [111 — 113] because of their similarity in the number of trans-membrane domains to bacteriorhodopsin. Such models prove to be useful for gaining further insight into the structure and function of receptors. However, their value for ligand design is limited at atomic resolution such models are far from reality. 

N] Vpu (2-30), trans-membrane domain of virus protein u from HIV-1 36 18 408... 

Length of trans-membrane helices. The length of the single hydrophobic trans-membrane domain of cyt / near its COOH terminus is 20 residues, compared to 23, 19, 23, and 23 residues predicted for the four helices of cyt 25-26 residues for cyt b-559 a, P, 23-24 for bacteriorhodopsin (35), and an average of approximately 26 for the photosynthetic reaction center (e.g., ref. 40). The shorter length of the predicted /rfl 5-membrane span of cyt / and b may be a result of assuming that all charged residues are excluded from the bilayer. 

Retinal is parallel to the surface of membrane and is attached to a lysin residue at position 296 in the seventh trans membrane domain. 

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