Total internal reflection fluorescence dynamic anisotropy

Rotational dynamics of a fluorescent dye adsorbed at the interface provides useful information concerning the rigidity of the microenvironment of liquid-liquid interfaee in terms of the interfacial viscosity. The rotational relaxation time of the rhodamine B dye was studied by time-resolved total internal reflection fluorescent anisotropy. In-plane... 

Rotational dynamics of a fluorescent dye adsorbed at the interface provides useful information concerning the rigidity of the microenvironment of liquid-liquid interface in terms of the interfacial viscosity. The rotational relaxation time of the rhodamine B dye was studied by the time-resolved total internal reflection fluorescent anisotropy. In-plane rotational relaxation time of octadecylrhodamine B cation was evaluated under the presence or absence of a surfactant [26]. Table 2.8 shows that by adding a surfactant, the relaxation time and the interfadal viscosity increased. Anionic surfactants SDS and HDHP (hydrogen dihexadecylphosphate) were more effective in reducing the rotational motion, because of the electrostatic interaction. HDHP with double long chains hindered the interfacial rotation more [40]. 

In 1995, Michael and Benjamin had suggested that picosecond time-resolved fluorescence following the excitation of amphiphilic solutes adsorbed at the interface could be used to probe the width of the interface [33], In 1999, Ishizaka et al. performed the first experiment to probe the interfacial roughness of the H2O-CCI4 and the H20-1,2-DCE interface by measuring the dynamic fluorescence anisotropy of sulforhodamine 101 (SRIOI) using time-resolved total internal reflection (TIR) fluorimetry [34],... 

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