TOPICAL metal binding site

Relaxation Properties A knowledge of the spin relaxation properties is of interest for at least two reasons. The first is related to the use of acquisition parameters for optimum signal-to-noise acquisition times and the second is to use the relaxation data to provide important information concerning motional dynamics in and around the metal binding site. Unfortunately, to our knowledge there has been no comprehensive Cd NMR relaxation study on macromolecular systems, which limits our discussion of this topic to early, preUminaiy investigative studies dating back to the mid to late 80s. 

The active-site metal ion plays an accepted role in the catalytic function of IN, although its role in inhibition is poorly understood. The role of the metal ion in inhibition has been a topic of speculation since the first inhibitors began to appear in the literature in 1993. Early work identified some DNA-binding and DNA-intercalating molecules as IN inhibitors (18). These early inhibitors include doxorubicin (Fig. 2,1), caffeic acid phenethyl ester (CAPE, Fig. 2,2), and quercetin (Fig. 2,3). This work established, however, that there was no clear relationship between DNA-binding or DNA-intercalating activities and inhibition. It was noted that active inhibitors contained polyhydroxylated aromatic regions that... 

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