Top-down mass spectrometry

Han, X., Jin, M., Breuker, K., McLafferty, F.W. (2006). Extending top-down mass spectrometry to proteins with masses greater than 200 kilodaltons. Science 314, 109-112. 

LeDuc, R.D., Taylor, G.K., Kim, Y.B., Januszyk, T.E., Bynum, L.H., Sola, J.V., Garavelli, J.S., Kelleher, N.L. (2004). ProSight PTM an integrated environment for protein identification and characterization by top-down mass spectrometry. Nucleic Acids Res. 32, W340-W345. 

X. Han, M. Jin, K. Breuker, and F. W. McLafferty. Extending Top-Down Mass Spectrometry to Proteins with Masses Greater Than 200 Kilodaltons. Science, 314(2006) 109-112. 

Siuti N, KeUeher NL. Decoding protein modifications using top-down mass spectrometry. Nat. Methods 2007 4 817-821. 

Zhai H, Han X, Breuker K, et al. (2005). Consecutive ion activation for top down mass spectrometry improved protein sequencing by nozzle-skimmer dissociation. Anal. Chem. 77(18) 5777-5784. 

Ge, Y Lawhorn, B.G. Elnaggar, M. Sze, S.K. Begley, T.P. McLafferty, F.W. Detection of four oxidation sites in viral prolyl-4-hydroxylase hy top-down mass spectrometry. Protein Science 2003,12, 2320-2326. 

Patrie, S.M. Ferguson, J.T. Robinson, D.E. Whipple, D. Rother, M. Metcalf, W.W. Kelleher, N.L. Top-down mass spectrometry of < 60 kDa proteins from Methanosarcina acetivorans using quadrupole FTMS with automated octopole CAD. Mol. Cell. Proteomics 2006, 5, 14-25. 

Figure 5 Top-down mass spectrometry analysis of CCL2. (A) Intact mass of purified recombinant CCL2. Pyroglutamate at the amino terminus explains the mass discrepancy. (B) Fragmentation of CCL2 shows amino terminal pyroglutamate ions with a p-value of lOx 10 .

Wang, G., Abzalimov, R.R., Bobst, C.E., Kaltashov, I.A. (2013) Conformer-specific characterization of non-native protein states using hydrogen exchange and top-down mass spectrometry. Proc Natl Acd Sci USA, 110 (50), 20087-20092. 

The lift TOP mass spectrometers [50, 57] can be used for top-down mass spectrometry of whole proteins. Although this has been achieved in practice, it either yields huge numbers of very small peptide fragments (e.g., rmmonium ions) or it sequences the termini of the protein nicely, but does not yield the large complementary ions needed for good top-down analyses. However, this top- down mass spectrometry parameter space has not yet been fully explored for TOP/TOF instruments. 

McFarland MA, Andrzejewski D, Musser SM, Callahan JH. Platform for identification oiSalmonella serovar differentiating bacterial proteins by top-down mass spectrometry S. Typhimuri-um vs S. Heidelberg. Anal Chem. 2014 86(14) 6879 86. 

Bacterial Identification at the Serovar Level by Top-Down Mass Spectrometry... 

Zamdborg L, LeDuc RD, Glowacz KJ, Kim Y-B, Viswanathan V, Spaulding IT, Early BP, Bluhm EJ, Babai S, KelleherNL. ProSight PTM 2.0 improved protein identification and characterization for top down mass spectrometry. Nucleic Acids Res. 2007 35 W701-6. 

Pesavento, j. J. Kim, Y. B. Taylor, G. K. Kelleher, N. L. Shotgun annotation of histone modifications A new approach for streamlined characterization of proteins by top down mass spectrometry. J. Am Chem. 

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