Tomato juice-glucose-fructose-malate medium

2 Tomato Juice-Glucose-Fructose-Malate Medium 

Mix 1 g liver extract with 100 mL water for at least 30 min. Filter through Whatman no.l filter paper prior to adding to the medium. 

Select a commercially processed tomato juice that contains only juice and salt (no preservatives and not from concentrate) or prepare juice from fresh tomatoes. Centrifuge 250 mL of the juice at 3000 x gfor 30min and decant, saving the supernatant (serum). Serum can be stored in small volumes at —20°C/—4 F until used. 

Add the filtered liver extract from step 1 to the ingredients specified in step 3 and adjust to pH 5.5 using 50% v/v H3PO4 and/or 6M KOH. 

To prepare a solidified medium, add 20 g agar prior to autoclaving at 121°C/250°Ffor 15min. 

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The media outlined for the cultivation of lactic acid bacteria are the apple juice Rogosa medium (King and Beelman, 1986) and the tomato juice-glucose-fructose-malate medium (Izuagbe et al., 1985). These media use either apple juice or tomato juice serum to provide the so-called tomato juice factor (Section 2.3). Liver extract or concentrate has a number of vitamins that improves bacterial growth and is available from Sigma Chemical Company. Both media can be made selective against Sac-charomyces by the addition of cycloheximide. 

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See also in sourсe #XX -- [ ]

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