Apple juice Rogosa medium

The media outlined for the cultivation of lactic acid bacteria are the apple juice Rogosa medium (King and Beelman, 1986) and the tomato juice-glucose-fructose-malate medium (Izuagbe et al., 1985). These media use either apple juice or tomato juice serum to provide the so-called tomato juice factor (Section 2.3). Liver extract or concentrate has a number of vitamins that improves bacterial growth and is available from Sigma Chemical Company. Both media can be made selective against Sac-charomyces by the addition of cycloheximide. 

The heterofermentation-arginine medium (Section 13.6.3) was developed by Pilone et al. (1991) for use in characterizing different physiological traits of lactic acid bacteria (Sections 15.4.1 and 15.4.5.2). 

Mix Ig liver extract or concentrate with 100 mL distilled water for at least 30 min. Filter through Whatman no.l filter paper. 

Add the filtered liver extract to the ingredients specified in step 2 and acljust to pH 4.5 using 50% v/v H3PO4 and/or 6M KOH. 

If the medium is to be selective against Saccharomyces, mix 100 mg cycloheximide with 10 mL distilled water and filter sterilize through an 

---