TNFa-induced NF-kB activation

Erlejman AG, Jaggers G, Fraga CG, Oteiza PI. 2008. TNFa-induced NF-kB activation and cell oxidant production are modulated by hexameric procyanidins in Caco-2 cells. Arch Biochem Biophys 476 186-195. 

Varfolomeev E, Goncharov T, Fedorova AV et al (2008) c-IAPl and C-IAP2 are critical mediators of Tumor Necrosis Factor alpha (TNFa)-induced NF-kB activation. J Biol Chem 283(36) 24295-24299... 

Schumacher, M., Cerella, C., Fifes, S., Chateauvieux, S., Morceau, F., (aspars, M., Dicato, M., and Diederich, M. (2010) Heteronemin, a spongean sesterterpene, mhibits TNFa-induced NF-kB activation through proteasome inhibition and induces apoptotic cell death. Biochem. Pharmacol, 79, 610-622. 

Varfolomeev E, Blankenship JW, Wayson SM et al (2007) IAP antagonists induce autoubiqui-tination of c-IAPs, NF-kB activation, and TNFa-dependent apoptosis. Cell 131(4) 669-681... 

Ubiquitin-proteasome pathway, involved in the degradation of tumor growth proteins, plays an important role in the treatment of cancer. Physalin B (6), the major steroidal lactone of Physalis angulata, is known to produce the ubiquitinated protein accumulation and inhibit the TNFa-induced activation of NF-KB in DLD-1 4Ub-Luc cell assay. Physalin B does not inhibit purified proteasome catalytic activities but interferes with the cellular catalytic activities of the proteasome proteins at 4—8-fold high concentration, which is required to produce significant rise in bioluminescence and ubiquitinated protein accumulation in DLD-1 4Ub-Luc cells. The results indicate that physalin B is cytotoxic and works as an apoptotic triggering agent in DLD-1 4Ub-Luc cells [95]. 

---