Tissue regeneration differentiated cell-scaffold systems

This section aims to illustrate some of the most common systems normally associated with a tissue regeneration goal. Thus, such systans are incorporated into the scaffolds or constitute the scaffolds themselves or aim at the delivery of factors affecting cell fate growth factors or differentiation factors. 

Cell-based scaffold systems for bladder regeneration that are based on the use of isolated urothelial and smooth muscle cells, which are the key cellular components of the native bladder, have shown promise in early studies. In order to engineer bladder tissue in vitro, these cell types can be cultured and expanded in vitro, seeded on scaffolds, and allowed to adhere to the scaffold. They then form cellular structures on the scaffold, and this construct can be implanted into the patient. It is hypothesized that the tissue engineered 3D bladder, will exhibit fully differentiated cell populations after implantation in vivo and the presence of these cells will reduce the inflammatory immune response to the matrix as well as prevent graft contraction and shrinkage. 

---