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Tissue culture selection technology

The development of selection technology is necessary in order to derive cell lines with specific traits, such as herbicide resistance, from tissue culture. Figure 9 depicts the somatic cell selection process using cell culture techniques. At the bottom left is a flask containing a suspension of alfalfa cells. These cells have been cultured for 4-8 weeks and then sieved to yield very small cell clumps which will be used for selection in vitro. [Pg.484]

Current technologies provide for availability of cell culture models from a selection of the significant tissue barriers as either primary cell culture systems or continuous cell line cultures. A number of endothelial and epithelial cell systems can now be grown onto permeable supports as monolayer systems to facilitate transcellular transport. These cell culture models provide powerful tools for the pharmaceutical chemist to characterize fundamental cellular transport mechanisms at the biochemical and molecular levels and effective screening systems to facilitate appropriate drug design and development. Moreover, they all have applications in... [Pg.118]


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