Tinospora malabarica

Tinospora malabarica (Lam.) Miers ex Hook. f. Thoms. Monimlaceae 1... 

The effects of dry stem crude extracts (DSCE) of Tinospora cordifolia and a related plant Tinospora malabarica have been shown to contain polyclonal B cell activators which are assumed to be polysaccharides. The DSCE were prepared by boiling the powdered stems in water followed by treatment with 10% trichloroacetic acid and filtration. The supernatant was further precipitated with acetone, and then fractionated on Sephadex G-200 and Sephacryl S-400 columns. The effects of these fractions were evaluated by culturing lymphocytes in vitro for 48 h in a 5% CO2 environment. Proliferation was estimated by pulsing 2x 10 cells with 1 iCi H-thymidine for 16 h and its incorporation measured by liquid scintillation counting. It was found that DSCE was mitogenic for lymphocytes from lymph node and spleen, while thymocytes, bone marrow cells and peripheral blood cells responded poorly [15]. 

The only iuroquinoline alkaloid ever isolated Irom a Menispeimacea was obtained from Tinospora malabarica when Bowen et al. were studying the anti-malarial properties of this plant (76). It was isolated before from Evodia spp. and Orixa spp. (Rutaceae) and Flindersia spp. (Rutaceae) (77). Scheme 24 shows the biogenetic pathway of kokusaginine. 

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