Three step purification of a recombinant antigen binding fragment

Three step purification of a recombinant antigen binding fragment 

This example demonstrates a three stage purification strategy in which the same purification principle is used in two different modes in the capture and polishing step IEX for capture, HIC for intermediate purification and IEX for the polishing step. The objective of this purification was to scale up the purification for use as a routine procedure. 

A more detailed description of this work can be found in Application Note 18-1111-23. 

Recombinant antigen binding fragment (Fab) directed against HTV gp-120. 

The anti-gp 120 Fab was expressed in the periplasm of the E. coli strain BM170 MCT61. E. coli pellets were stored frozen after being harvested and washed once. 

---