Thermodynamics protein binding

C. M. Reyes and P. A. Kollman, Structure and thermodynamics of RNA-protein binding ... 

Solution equilibria for gadolinium imaging agents have been studied with consideration for pharmacokinetic, protein binding, elimination, and safety aspects of the dmgs. The thermodynamic stability constant, Kq defined by equation 7.4 must be large for clinically viable agents. Some Kq l data are listed in Table 7.3. 

Thus structural background suggests that the bound cyt c ccp adduct may actually consist of a distribution of structures. In this section, we consider the thermodynamics of binding cyt c and ccp, both for the native proteins, from different species, and proteins incorporating single site replacements, as prepared by site directed mutagenesis. 

In this study the physical parameters involved in interaction of a major class of meat flavorants, methyl pyrazines, with soy proteins were determined at meat roasting temperatures. Beef diffusate, the water soluble, low molecular weight fraction that constitutes about IX of beef, was shown to contain the necessary precursors to obtain a desirable, thermally generated meat aroma (8). Diffusate was heated under controlled conditions and generated volatiles were transferred to a gas chromatograph for separation and quantitation. Methyl pyrazines, either from heated diffusate or from standard solutions, were measured in the presence of purified soy proteins and the thermodynamics of binding were determined. 

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